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Micromotor Enhanced microarray technology for Protein Detection

SMALL 2014年第13期10卷 2542-2548页

作者： Morales-Narvaez, Eden Guix, Maria Medina-Sanchez, Mariana Mayorga-Martinez, Carmen C. Merkoci, Arben Catalan Inst Nanosci & Nanotechnol Nanobioelect & Biosensors Grp Barcelona 08193 Spain Univ Politecn Cataluna ESAII Dept Barcelona 08028 Spain Autonomous Univ Barcelona UAB Barcelona 08193 Spain ICREA Barcelona Spain

Microengines meet microarray technology. The transport of a target molecule toward a sensing surface can play a critical role in biodetection performance. The mixing induced by the motion of self-propelled micromotors can assist such transport and consequently enhance the performance of surface-based biosensing systems, particularly in microarray-based immunosensing that can be extended to DNA and cell analysis. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

关键词： micromotors microarray technology protein detection surface-based biosensing

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Phenotype microarray technology and its application in industrial biotechnology

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BIOtechnology LETTERS 2014年第6期36卷 1153-1160页

作者： Greetham, Darren Univ Nottingham Sch Biosci Nottingham LE12 6RD Leics England

Phenotype microarray (PM) technology provides an insight into the metabolic profiling of microbial cells within 96-well plate system. The PM assay allows for cells to be assessed for utilisation of nutrients or sensitivity to toxic compounds. The assay utilises a redox sensitive tetrazolium dye which becomes irreversibly reduced upon detection of cellular metabolic output, detection is synchronous with a colour change from colourless to purple. Output from PM technology can be measured visually or quantified by reader the absorbance in each well. PM technology has highlighted differences in growth requirements, nutrient utilisation, sensitivity to toxins, and genetic diversity in bacteria, fungi and mammalian cells.

关键词： Live cells Metabolic output Metabolic profiling microarray technology Phenotypic microarray

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Affinity profiling using the peptide microarray technology: A case study

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ANALYTICAL BIOCHEMISTRY 2007年第1期363卷 108-118页

作者： Tapia, Victor Bongartz, Juliane Schutkowski, Mike Bruni, Nicole Weiser, Armin Ay, Bernhard Volkmer, Rudolf Or-Guil, Michal Charite Univ Med Berlin Inst Med Immunol Mol Lib & Recognit Grp D-10115 Berlin Germany Humboldt Univ Inst Theoret Biol D-10115 Berlin Germany JPT Peptide Technol GmbH D-12489 Berlin Germany

Little about the reliability of measurements obtained using synthetic peptide microarrays is known. We report results from a study on the quantitative reliability of microarrays manufactured by robot-supported immobilization of presynthesized peptides for different microarray platforms. Technological precision is assessed for inter- and intra-device readout comparisons. Correlations between measured signals and known dissociation constants using a phenomenological model derived from the mass action law are discussed. Special emphasis is on discussing the pitfalls of high-throughput affinity measurements. We show that the quantitative determination of binding affinities is prone to be biased toward a mean affinity of around 10(-7) M, while the classification of peptides into either "binders" or "nonbinders" provides very high prediction accuracy. The experimental requirements needed to obtain reliable binding affinity predictions are discussed. (c) 2007 Elsevier Inc. All rights reserved.

关键词： microarray technology synthetic peptide arrays mass action law precision accuracy binding affinity

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A method adapting microarray technology for signature-tagged mutagenesis of Desulfovibrio desulfuricans G20 and Shewanella oneidensis MR-1 in anaerobic sediment survival experiments

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APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2005年第11期71卷 7064-7074页

作者： Groh, JL Luo, QW Ballard, JD Krumholz, LR Univ Oklahoma Dept Bot & Microbiol Norman OK 73019 USA Univ Oklahoma Dept Microbiol & Immunol Hlth Sci Ctr Oklahoma City OK 73019 USA

Signature-tagged mutagenesis (STM) is a powerful technique that can be used to identify genes expressed by bacteria during exposure to conditions in their natural environments. To date, there have been no reports of studies in which this approach was used to study organisms of environmental, rather than pathogenic, significance. We used a mini-Tn10 transposon-bearing plasmid, pBSL180, that efficiently and randomly mutagenized Desulfovibrio desulfuricans G20 in addition to Shewanella oneidensis MR-1. Using these organisms as model sediment-dwelling anaerobic bacteria, we developed a new screening system, modified from former STM procedures, to identify genes that are critical for sediment survival. The screening system uses microarray technology to visualize tags from input and output pools, allowing us to identify those lost during sediment incubations. While the majority of data on survival genes identified will be presented in future papers, we report here on chemotaxis-related genes identified by our STM method in both bacteria in order to validate our method. This system may be applicable to the study of numerous environmental bacteria, allowing us to identify functions and roles of survival genes in various habitats.

关键词： .procedures pools tags mini-Tn Shewanella oneidensis MR Desulfovibrio desulfuricans microarray technology Sediment Survival pBSL sediment-dwelling input and output sediment incubations STM method

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Synthesis of Clickable Coating Polymers by Postpolymerization Modification: Applications in microarray technology

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LANGMUIR 2016年第40期32卷 10284-10295页

作者： Sola, Laura Damin, Francesco Gagni, Paola Consonni, Roberto Chiari, Marcella CNR Ist Chim Riconoscimento Mol Via Mario Bianco 9 I-20131 Milan Italy CNR Ist Studio Macromol Via Corti 12 I-20133 Milan Italy

In this paper, we report on the postpolymerization modification (PPM) of a polymer to introduce new functionalities that enable click chemistry reactions for microarray applications. The parent polymer, named copoly(DMA-NAS-MAPS), is composed of N,N-dimethylacrylamide (DMA), a monomer that self-adsorbs onto different materials through weak interactions such as hydrogen bonding or van der Waals forces, 3-(trimethoxysilyl)propyl methacrylate (MAPS) that strengthens the stability of the coating through the formation of covalent bonds with siloxane groups on the surface to be coated, and N-acryloyloxysuccinimide (NAS), an active ester group, highly reactive toward nucleophiles, which enables bioprobe immobilization. This copolymer has been widely exploited to coat surfaces for microarray applications but exhibits some limitations because of the potential hydrolysis of the active ester (NHS ester). The degradation of the NHS ester hampers the use of this coating in some situations, for example, when probe immobilization cannot be accomplished through a microspotting situation, but in large volumes, for example, in microchannel derivatization or micro-/nanoparticle functionalization. To overcome the limitations of NHS esters, we have developed a family of polymers that originate from the common copolymer precursor, by reacting the active ester contained in the polymer chain with a bifunctional amine. In particular, the functional groups introduced in the polymer using PPM enable click chemistry reactions such as azide/alkyne or thiol/maleimide "click" reactions, with suitably modified biomolecules. The advantages of such reactions are quantitative yields, orthogonality of functional groups, and insensitivity of the reaction to pH. The new click functionalities, inserted with quantitative yields, improve the stability of the coating, enabling the attachment of biomolecules directly from a solution and avoiding the spotting of reduced volumes (pL) of probes. Finally, we hav

关键词： SURFACE coatings POLYMERS POLYMERIZATION CLICK chemistry microarray technology HYDROGEN bonding

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Fluorescence analysis in microarray technology

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MICROCHIMICA ACTA 2005年第1-2期151卷 1-21页

作者： Nagl, S Schaeferling, M Wolfbeis, OS Univ Regensburg Inst Analyt Chem Chemo & Biosensors D-93040 Regensburg Germany

Fluorescence has been the preferred choice for data quantification in biomedical microarray formats since their earliest days. As much as the formats have grown and evolved over the years, the methods in optical analysis have become ever more sophisticated and complex in order to produce more and better output. This review will provide an insight into the most common methods and the state-of-the-art of all areas in microarray fluorescence analysis. Starting with an overview on microarray formats with a focus on their demands on the readout, the most common and useful organic fluorescent stains are discussed before proceeding on to other approaches;the use of semiconductor nanocrystals (quantum dots), polymer and silica nanoparticles and fluorescent proteins. Ways to enhance the intrinsically low signal on biochips have become increasingly important as they offer a sound approach towards the detection of low concentration sample content. The three main categories are presented: amplification using DNA, enzymes, and dendrimers. As much diversity as on the microarrays themselves can be found at the detection device. Standard optical microarray detectors, and non-standard methods using fluorescence anisotropy, fluorescence lifetime imaging (FLIM) and fluorescence resonance energy transfer (FRET), and their advantages and disadvantages are discussed.

关键词： microarray technology fluorescence analysis signal amplification optical methods fluorescence lifetime imaging

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A rapid and high-throughput method for the determination of serum uric acid based on microarray technology and nanomaterial

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LUMINESCENCE 2017年第5期32卷 730-734页

作者： Li, Xiaohua Bai, Yunfeng Feng, Feng Zhang, Zhujun Shanxi Datong Univ Sch Chem & Environm Engn Datong 037009 Shanxi Peoples R China Shaanxi Normal Univ Sch Chem & Chem Engn Xian 710119 Peoples R China

Serum uric acid (SUA) is a new therapeutic target for non-alcoholic fatty liver disease (NAFLD). In this study, we introduced a chemiluminescence (CL) method combined with microarray technology and a simple fabrication procedure to obtain a highly sensitive SUA probe based on a mesoporous metal oxide nanomaterial. The high-throughput method was based on the generation of H2O2 from SUA by immobilized uricase and its measurement by a CL reaction catalyzed by mesoporous metal oxide nanomaterials. The CL probe was designed for SUA The linear range of the uric acid concentration was 0.6-9M and the detection limit was 0.1M. In comparison with the other SUA detection techniques, this method has the advantages of a low detection limit, high sensitivity and simplicity. A new sensitive high-throughput approach was obtained for the determination of SUA.

关键词： high-throughput microarray technology nanomaterial serum uric acid

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Prostasin, a potential serum marker for ovarian cancer: Identification through microarray technology

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JOURNAL OF THE NATIONAL CANCER INSTITUTE 2001年第19期93卷 1458-1464页

作者： Mok, SC Chao, J Skates, S Wong, KK Yiu, GK Muto, MG Berkowitz, RS Cramer, DW Harvard Univ Lab Gynecol Oncol Brigham & Womens Hosp Sch MedDept Obstet Gynecol & Reprod Biol Boston MA 02115 USA Med Univ S Carolina Dept Biochem & Mol Biol Charleston SC 29425 USA Dana Farber Canc Inst Gillette Ctr Womens Canc Boston MA 02115 USA Massachusetts Gen Hosp Ctr Biostat Boston MA 02114 USA Texas Childrens Hosp Baylor Coll Med Dept Pediat Houston TX 77030 USA Harvard Univ Sch Med Brigham & Womens Hosp Dept Obstet Gynecol & Reprod Biol Cambridge MA 02138 USA Dana Farber Canc Inst Gillette Ctr Womens Canc Boston MA USA

Background: Screening biomarkers for ovarian cancer are needed because of its late stage at diagnosis and poor survival. We used microarray technology to identify overexpressed genes for secretory proteins as potential serum biomarkers and selected prostasin, a serine protease normally secreted by the prostate gland, for further study. Methods: RNA was isolated and pooled from three ovarian cancer cell lines and from three normal human ovarian surface epithelial (HOSE) cell lines. Complementary DNA generated from these pools was hybridized to a microarray slide, and genes overexpressed in the cancer cells were identified. Real-time quantitative polymerase chain reaction was used to examine prostasin gene expression in ovarian cancer and HOSE cell lines. Anti-prostasin antibodies were used to examine prostasin expression and to measure serum prostasin by an enzyme-linked immunosorbent assay in 64 case patients with ovarian cancer and in 137 control subjects. Previously determined levels of CA 125, an ovarian cancer marker, were available from about 70% of all subjects. All statistical tests were two-sided. Results: Prostasin was detected by immunostaining more strongly in cancerous ovarian epithelial cells and stroma than in normal ovarian tissue. The mean level of serum prostasin was 13.7 mug/mL (95% confidence interval [CI] = 10.5 to 16.9 mug/mL) in 64 case patients with ovarian cancer and 7.5 mug/mL (95% CI = 6.6 to 8.3 mug/mL) in 137 control subjects (P < .041, after adjustment for the subject's age, year of collection, and specimen quality). In 14 of 16 case patients with both preoperative and postoperative serum samples, postoperative prostasin levels were statistically significantly lower than preoperative levels (P =.004). In 37 case patients with nonmucinous ovarian cancer and in 100 control subjects for whom levels of CA 125 and prostasin were available, the combination of markers gave a sensitivity of 92% (95% CI = 78.1% to 98.3%) and a specificity of 94%

关键词： Carcinoma Gene Expression Profiling Mass Screening Neoplasm Proteins 癌普查肿瘤蛋白质类寡核苷酸序列分析卵巢肿瘤肿瘤标记生物学 microarray technology Prostate Neoplasm Proteins Carcinoma Ovary Cancer prostasin Serum Markers PRSS8 gene Mass Screening ovarian cancer cell line Gene Expression Profiling Tumor markers

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Use of microarray technology to profile gene expression patterns important for reproduction in cattle

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REPRODUCTION IN DOMESTIC ANIMALS 2008年第2期43卷 359-367页

作者： Evans, A. C. O. Forde, N. O'Gorman, G. M. Zielak, A. E. Lonergan, P. Fair, T. Univ Coll Dublin Sch Agr Food Sci & Vet Med Vet Sci Ctr Dublin 4 Ireland Univ Coll Dublin Conway Inst Dublin 4 Ireland Wroclaw Univ Environm & Life Sci Fac Biol & Anim Sci Inst Anim Breeding Wroclaw Poland

Fertility in cattle is a major component of many agricultural enterprises and there is pressure to devise methods to improve this. A number of approaches are ongoing, one of which is to better understand the cellular and molecular events of the development of reproductive tissues and to use these as targets for developing new strategies. microarray technologies now allow us the potential to determine the transcriptional profile of expressed genes in a given tissue. This review focuses on the types of microarrays available for studies in cattle and concludes that genes associated with one or more of the cellular processes of cell survival/death, intracellular signalling, transcription and translation, cell division and proliferation and cellular metabolism are the main transcriptional pathways that control the development of ovarian follicles, oocytes, early embryos and the uterine endometrium about the time of the establishment of pregnancy.

关键词： farm enterprises Fertility cellular metabolism Genes master element Cellular processes Bos taurus microarray technology Gene Expression Profiling microarray

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DNA microarray technology for the microbiologist: an overview

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APPLIED MICROBIOLOGY AND BIOtechnology 2006年第2期73卷 255-273页

作者： Ehrenreich, Armin Univ Gottingen Inst Microbiol & Genet D-37077 Gottingen Germany

DNA microarrays have found widespread use as a flexible tool to investigate bacterial metabolism. Their main advantage is the comprehensive data they produce on the transcriptional response of the whole genome to an environmental or genetic stimulus. This allows the microbiologist to monitor metabolism and to define stimulons and regulons. Other fields of application are the identification of microorganisms or the comparison of genomes. The importance of this technology increases with the number of sequenced genomes and the falling prices for equipment and oligonucleotides. Knowledge of DNA microarrays is of rising relevance for many areas in microbiological research. Much literature has been published on various specific aspects of this technique that can be daunting to the casual user and beginner. This article offers a comprehensive outline of microarray technology for transcription analysis in microbiology. It shortly discusses the types of DNA microarrays available, the printing of custom arrays, common labeling strategies for targets, hybridization, scanning, normalization, and clustering of expression data.

关键词： GENE-EXPRESSION DATA MESSENGER-RNA DECAY HEAT-SHOCK RESPONSE ESCHERICHIA-COLI BACILLUS-SUBTILIS CDNA microarray GLOBAL ANALYSIS OLIGONUCLEOTIDE microarray TRANSCRIPTIONAL RESPONSE DIFFERENTIAL EXPRESSION cDNA Array Genome RNA Decay GLOBAL ANALYSIS DNA microarray technology bacterial metabolism microarray technology Bacillus subtilis synthetic data

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