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High-throughput fluorescence quantification method based on inner filter effect and fluorescence imaging analysis

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2024年 318卷 124422页

作者： Li, Wanxiang Fu, Yuchao Liu, Tianyuan Li, Haochen Huang, Meizhen Shanghai Jiao Tong Univ Sch Elect Informat & Elect Engn Dept Instrument Sci & Engn Shanghai 200240 Peoples R China Shanghai Jiao Tong Univ Sch Elect Informat & Elect Engn Dept Elect Engn Shanghai 200240 Peoples R China

The application of the inner filter effect (IFE) in fluorescent substance determination is gaining popularity. In this paper, a theory of the fluorescence distribution along with the excitation light path is derived from our previous research about the spatial micro-element method. According to the relationship between the summation of fluorescence intensities along the vertical direction at a certain position on the excitation light path and the position, a high-concentration and wide-range fluorescent substance quantification method based on the IFE and fluorescence imaging analysis is proposed. Correspondingly, a high-throughput fluorescent substance quantification detection system is constructed. In order to validate the method, solutions of rhodamine B in different concentrations are used for principle validation, concentration prediction, and experimental investigation on the influence of integration time and lens distortion. The high-throughput system enables the simultaneous measurement of six samples, realizing the high-concentration and wide-range quantification of rhodamine B (100-600 mg/L) with high precision (R2 = 0.9992, MRE = 2.34 %). By setting the filter wheel, the system can measure the concentration of fluorescent substances with different emission wavelengths. The improvement of experimental device is expected to reduce the single sample capacity to tens of microliters and increase the overall sample quantity to tens or even hundreds. The proposed method and system are beneficial to fluorescence measurement in fields such as biomedicine and dye research and to the improvement of high-throughput fluorescence quantitative PCR instruments.

关键词： Fluorescence Inner filter effect (IFE) High -throughput Fluorescence imaging quantification method

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Trust-based Access Control Model with quantification method for Protecting Sensitive Attributes

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INTERNATIONAL JOURNAL OF ADVANCED COMPUTER SCIENCE AND APPLICATIONS 2022年第2期13卷 695-707页

作者： Salji, Mohd Rafiz Udzir, Nur Izura Ninggal, Mohd Izuan Hafez Sani, Nor Fazlida Mohd Ibrahim, Hamidah Univ Teknol MARA Fac Informat Management Shah Alam Malaysia Univ Putra Fac Comp Sci & Informat Technol Seri Kembangan Malaysia

The prevailing trend of the seamless digital collection has prompted privacy concerns to the organization. In enforcing the automation of privacy policies and laws, access control has been one of the most devoted subjects. Despite the recent advances in access control frameworks and models, there are still issues that hinder the implementation of successful access control. This paper illustrates the problem of the previous model which typically preserves data without explicitly considering the protection of sensitive attributes. This paper also highlights the drawback of the previous works which provides inaccurate calculation to specify user's trustworthiness. Therefore, a trust-based access control (TBAC) model is proposed to protect sensitive attributes. A quantification method that provides accurate calculation of the two user properties is also proposed, namely: seniority and behaviour to specify user's trustworthiness. Experiment have been conducted to compare the proposed quantification method and the previous quantification methods. The result shows that the proposed quantification method is stricter and accurate in specifying user's trustworthiness as compared to the previous works. Therefore, based on the result, this study resolves the issue of specifying the user's trustworthiness. This study also indicates that the issue of protecting sensitive attributes has been resolved.

关键词： Access control trust-based access control quantification method sensitive attributes privacy privacy protection

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Accuracy Assessment of quantification method of Cellulose Nano-Fiber in Nickel Plating Film Using Image Analysis 20

Accuracy Assessment of Quantification Method of Cellulose Na...

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20th International Conference on Electronics Packaging (ICEP)

作者： Iioka, M. Shohji, I Kobayashi, T. Gunma Univ Grad Sch Sci & Technol Kiryu Gumma Japan

ISBN: (纸本)9784991191107

We have been studying about nickel and cellulose nano-fiber (Ni-CNF) composite plating to provide functionality for Ni plating film used widely industrially. The aim of this study is accuracy assessment of the quantification method of CNF in nickel film using image analysis which has some accuracy issues reported in our previous study. quantification analysis by X-ray fluorescence (XRF) analysis with fundamental parameter method and by the image analysis method with binarized backscattered electron (BSE) images of film surface were conducted to obtained Ni-CNF composite film. From comparison of both results, it was revealed that CNF content ratios in film (codeposition rate) investigated by both methods have different trends. It seems that this result proceeds from morphology difference of the inside and the surface of film. Furthermore, investigation of suitable magnification of the BSE image for image analysis was carried out, and it was clarified that magnification does not affect quantifying results seriously. Based on results obtained by XRF analysis, the calibration constant for image analysis method was determined according to CNF concentration in plating bath.

关键词： Cellulose Nano-Fiber Composite Plating quantification method XRF Analysis Image Analysis

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The pulse waveform quantification method basing on contour and derivative

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COMPUTER methodS AND PROGRAMS IN BIOMEDICINE 2022年 220卷 106784-106784页

作者： Jin, Ji Zhang, Haiying Geng, Xingguang Zhang, Yitao Ye, Tianchun Chinese Acad Sci Inst Microelect Beijing 100029 Peoples R China Univ Chinese Acad Sci Sch Microelect Beijing 100049 Peoples R China

Objective: Pulse waveform contains abundant physiological and pathological information. The condition of surrounding arteries can be reflected sensitively by the contour and derivative changes of pulse waves. In order to express these changes objectively, the pulse wave needs to be ***: This study provides a novel quantification method for pulse waveform in the entire cardiac cy-cle. It involves two new quantification parameters k1 and k2 to display the waveform change caused by the superimposition of wave reflection in the systolic reflex period, which is the most significant changes period. In this method, multi parameters were fused by Kalman filter to obtain an optimal estimation, in-volving the new parameters and other parameters: k0 for the early systolic period, C1 and C2 for diastole period, and K for pulse ***: Use correlation analysis to verify the effectiveness of new parameters that the coefficient is 0.7 between them and the typical augmentation index (AIx). The quantification results of 462 single-cycle pulse waves have consistent change trends with aging in 25-75 different age groups. For respiration analysis, the correlation coefficients are all greater than 0.6, even achieved 0.8 in six multi-cycle data between Kalman optimal estimation and breath wave. Conclusion: This method has quantified the waveform change with physiological status, and these quan-tification parameters can display the detail of each period. Significance: It will be used to verify waveform recognition accuracy and has a vast potential to detect diseases.(c) 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license ( http://***/licenses/by-nc-nd/4.0/ )

关键词： Pulse wave quantification method quantification parameter Kalman filter Derivative

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Study on quantification method for dispersion and distribution of sphere-like particles and relationship with AC/DC breakdown strength in polymer nanocomposites

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IEEE TRANSACTIONS ON DIELECTRICS AND ELECTRICAL INSULATION 2020年第2期27卷 343-351页

作者： Qiang, Dayuan Wang, Y. Alhabill, F. N. Chen, G. Andritsch, T. Univ Southampton Dept Elect & Comp Sci Southampton SO17 1BJ Hants England

Polymer nanocomposites are more and more researched and employed as dielectrics in electrical power equipment. However, the bad dispersion and distribution of particles are often reported to deteriorate the dielectric properties of polymer nanocomposites. In most cases, researchers tend to use SEM/TEM images with imprecise descriptions to describe these two factors, and there was still no clear relationship between their quantified particle dispersion characteristics and dielectric properties of polymer nanocomposites. This work is to, first, propose a combined quantification method to estimate the dispersion and distribution of spherical/ellipsoidal particles/aggregates in polymer nanocomposites based on SEM images of epoxy SiO2 nanocomposites. Based on the proposed quantification method, epoxy nanocomposite with surface treated SiO2 shows overall better dispersion and distribution of particles/aggregates than those with untreated ones. The presence of agglomerations would lead to the enhancement of mobility of charge carriers and thus reduction of breakdown strength, which become more obvious with the growth of filler loadings. It is found that, in AC breakdown tests, dispersion and distribution of particles/aggregates show little influence on the reduction of AC breakdown strength. However, those should be the main factor which influences the DC breakdown strength in epoxy nanocomposites with a variation of filler loading concentrations.

关键词： nanotechnology dielectric breakdown silica epoxy resins electron microscopy quantification method

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An SMFL-based non-destructive quantification method for the localized corrosion cross-sectional area of rebar

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CORROSION SCIENCE 2021年 192卷 109793-109793页

作者： Qiu, Junli Zhang, Hong Zhou, Jianting Zhang, Weiping Tongji Univ Key Lab Performance Evolut & Control Engn Struct Minist Educ 1239 Siping Rd Shanghai 200092 Peoples R China Chongqing Jiaotong Univ State Key Lab Mt Bridge & Tunnel Engn 69 Xuefu Ave Chongqing 400074 Peoples R China Tongji Univ Dept Struct Engn 1239 Siping Rd Shanghai 200092 Peoples R China

The non-destructive quantitative evaluation of the rebar corrosion degree is an urgent problem to be solved for corroded reinforced concrete structures. In this study, a non-destructive quantification method for the rebar localized corrosion cross-sectional area was proposed based on the dynamic linkage mechanism of the induced magnetic and self-magnetic flux leakage fields of rebar. As verified by data from the experiment and the finite element simulation, this quantification method could eliminate the adverse influence of the magnetic property differences of the rebar. The results indicated that different quantitative indices for corrosion evaluation could be created based on this method, and an index created by two transformations with the same geometric characteristics had better quantification capability. The four quantitative indices I-1, I-2, I-3, and I-4 created in this study were all linearly related to the rebar localized corrosion cross-sectional area ratio S, and index I-3 had the relatively best quantitative capability and applicability. This quantification method provided an innovative solution for the non-destructive quantitative evaluation of rebar corrosion.

关键词： Rebar Localized corrosion cross-sectional area quantification method Self-magnetic flux leakage Non-destructive testing

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Research on Network Security Situation Assessment and quantification method Based on Analytic Hierarchy Process

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WIRELESS PERSONAL COMMUNICATIONS 2018年第2期102卷 1401-1420页

作者： Wang, Huan Chen, Zhanfang Feng, Xin Di, Xiaoqiang Liu, Dan Zhao, Jianping Sui, Xin Changchun Univ Sci & Technol Dept Comp Sci & Technol Changchun 132000 Jilin Peoples R China

In hierarchical network security situation assessment model, there are many problems, such as subjective index weight factor, large evaluation index system, large amount of calculation and low efficiency. A network security situation assessment model and quantification method based on AHP is proposed to solve this problem. The AHP analytic hierarchy process (AHP) is combined with the hierarchical model of situation assessment to simplify the situation assessment problem. The D-S evidence theory is used to fuse the fuzzy results of multi-source equipment to solve the problem of single information source and large deviation of accuracy. Through the construction of three evaluation indexes of risk situation, basic operation situation and damage situation, the problem of large evaluation index system and low evaluation efficiency is solved. AHP analytic hierarchy process is used to determine the weights of different index items, so as to avoid the subjectivity and randomness of weighting factors. The simulation results show that the model can reflect the security status of the network as a whole, and the situation assessment is accurate and can better serve the high-level decision-making.

关键词： AHP Situation assessment quantification method Network security

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A multiplex RNA quantification method to determine the absolute amounts of mRNA without reverse transcription

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ANALYTICAL BIOCHEMISTRY 2017年 539卷 96-103页

作者： Yokomori, Maasa Gotoh, Osamu Murakami, Yasufumi Fujimoto, Kenzo Suyama, Akira Univ Tokyo Grad Sch Arts & Sci Dept Life Sci Meguro Ku 3-8-1 Komaba Tokyo 1538902 Japan Tokyo Univ Sci Grad Sch Ind Sci & Technol Dept Biol Sci & Technol 2641 Yamazaki Noda Chiba 2788510 Japan Japan Adv Inst Sci & Technol Sch Mat Sci 1-1 Asahidai Nomi Ishikawa 9231292 Japan

We have developed a highly sensitive microarray-based method that determines the absolute amounts of mRNA in a total RNA sample in a multiplex manner without reverse transcription. This direct mRNA measurement promotes high-throughput testing and reduces bias in transcriptome analyses. Furthermore, quantification of the absolute amount of mRNA allows transcriptome analysis without common controls or additional, complicated normalization. The method, called Photo-DEAN, was validated using chemically synthesized RNAs of known quantities and mouse liver total RNA samples. We found that the absolute amounts of mRNA were successfully measured without the cDNA synthesis step, with a sensitivity of 15 zmol achieved in 7 h.

关键词： quantification method mRNA Multiplex assay Microarray Transcriptome Gene expression

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A quantification method for shale fracability based on analytic hierarchy process

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ENERGY 2016年第Part1期115卷 637-645页

作者： Sui, Lili Ju, Yang Yang, Yongming Yang, Yong Li, Aishan China Univ Min & Technol Beijing State Key Lab Coal Resources & Safe Min D11 Xueyuan RD Beijing 100083 Peoples R China North China Inst Sci & Technol Dept Fundamental Sci Beijing 101601 Peoples R China China Univ Min & Technol State Key Lab Geomech & Deep Underground Engn Xuzhou 221116 Peoples R China Shengli Oilfield Co SINOPEC Res Inst Geol Dongying 257000 Peoples R China Shengli Oilfield Co SINOPEC Res Inst Oil Prod Technol Dongying 257000 Peoples R China

A quantification method for evaluation of the fracability of shale is essential for optimizing hydraulic fracturing of shale gas reservoirs and enhancing shale gas recovery. To quantitatively evaluate the fracability, seven sets of shale cores are drilled from the reservoirs at different depths of an oilfield in the east of China. The influences of six fracability-related mechanical and physical characteristics of shale, i.e. brittleness, brittle mineral content, clay mineral content, cohesion, angle of internal friction, and unconfined compressive strength, are analyzed. A mathematical model taking account of significance of the influencing factors is proposed based on analytic hierarchy process (AHP) to evaluate the fracability according to their different effects on shale fracability. The analysis indicates that the fracability decreases with the increase of reservoir depths. The hydraulic fracturing tests of the shale cores are conducted to verify the accuracy of the quantification method. The fractal geometry is used to characterize the fracture degree of the shale. It is shown that a larger fractal dimension of the fracture network corresponds to a better fracability of shale. The more complex the fractures are, the larger the fracability of shale is. The experimental data coincide with the results of the proposed evaluation model. (C) 2016 Elsevier Ltd. All rights reserved.

关键词： Shale fracability quantification method Influencing factors Analytical hierarchy process (AHP) Experimental measurement Fractal description

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Development of an UHPLC-UV method for quantification of Three Phenyl 4-(2-oxo-3-alkylimidazolidin-1-yl)benzenesulfonates in CD-1® IGS Female Mouse Plasma

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JOURNAL OF ANALYTICAL CHEMISTRY 2024年第4期79卷 456-463页

作者： Alvarez, Atziri Corin Chavez Bouzriba, Chahrazed Bernier, Laureline Ouellette, Vincent Gagne-Boulet, Mathieu Pilote, Sylvie Gaudreault, Rene C. - Simard, Chantale Fortin, Sebastien Univ Laval Fac Pharm Quebec City PQ G1V 0A6 Canada Univ Laval CHU Quebec Quebec City PQ G1L 3L5 Canada Univ Laval Ctr Rech Inst Univ Cardiol & Pneumol Quebec Quebec City PQ G1V 4G5 Canada Univ Laval Fac Med Dept medecine Mol Quebec City PQ G1V 0A6 Canada

Phenyl 4-(2-oxo-3-alkylimidazolidin-1-yl)benzenesulfonates (PAIB-SOs) are new antimitotic prodrugs bioactivated by the enzyme CYP1A1 into phenyl 4-(2-oxo-3-imidazolidin-1-yl)benzenesulfonates (PIB-SOs) that are potently (nM) and selectively inhibiting the growth of CYP1A1-expressing breast cancer cells. The screening program identified three PAIB-SOs designated as CEU-835, CEU-934, and CEU-938 for further pharmacokinetic studies. A novel method for their quantification in CD-1 (R) IGS female mouse plasma using a Waters UHPLC ACQUITY Arc system was developed and validated. Their analytical measurements ranged from 7.3 to 1484 ng/mL. They exhibited linearity between 7.3 and 1484 ng/mL. They also showed good resolutions (2.21-4.99) and good theoretical plate numbers (14707-70580). Moreover, they were robust at wavelengths between 273 and 310 nm and sensitive from 2 to 62 AU/ng mL(-1). This new method is indispensable to assess PAIB-SOs half-life in mouse plasma and for the quantification of PAIB-SOs in other biological matrices.

关键词： detection and validation method quantification method phenyl 4-(2-oxo-3-alkylimidazolidin-1-yl)benzenesulfonates PAIB-SOs CEU-835 CEU-934 CEU-938

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