Infectious bursal disease virus (IbDV) causes immunosuppression in chickens, with distinctive bursal lesions. Genomic sequence information is essential to understand the epidemiology of antigenically and/or pathogenic...
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Infectious bursal disease virus (IbDV) causes immunosuppression in chickens, with distinctive bursal lesions. Genomic sequence information is essential to understand the epidemiology of antigenically and/or pathogenically variable IbDV strains. The present study investigated the full-length coding sequences of genome segments A and b of IbDV circulating in Thailand during 2010-2015. Five clinical samples of pooled bursas obtained from five commercial chicken farms experiencing problems with IbD in five different provinces of Thailand were identified by using a one-step reverse transcriptase polymerase chain reaction. The full-length coding sequences of genome segments A and b of three and five isolates, respectively, were successfully characterized. Phylogenetic analysis showed that two groups of IbDV were circulating in Thailand. The first group was similar to strain HLJ-504, genotype A3b3, isolated in China, but it had some minor variations in both genome segments A and b. The second group, which had a recombination event in segment A, was clustered with genotype A1b1. Its segment A originated from the intermediate vaccine W2512-like strain and isolate 9109, whereas segmentb shared characteristics of a classical strain without recombination events. This study characterized the full-length coding sequences of genome segments A and b of Thai IbDV and demonstrated the presence of recombinant IbDV in Thailand for the first time. Therefore, the molecular characterization of the full-length coding sequences of both genome segments of IbDV is essential to monitor the emergence of new variant viruses, which is important for effective control and appropriate vaccine development.
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