Colorectal cancer (CRC) is the most common gastrointestinal malignancy, with its recurrence and metastasis significantly affecting patient survival. Circular RNAs (circRNAs), a novel class of noncoding RNAs, have emer...
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Colorectal cancer (CRC) is the most common gastrointestinal malignancy, with its recurrence and metastasis significantly affecting patient survival. Circular RNAs (circRNAs), a novel class of noncoding RNAs, have emerged as crucial contributors to CRC pathogenesis. However, the role of circEIF3I in CRC metastasis remains unclear. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to assess circEIF3I, microRNA (miR)-328-3p, and NCAPH expression. CRC cell migration and invasion were determined via Transwell assays. Western blot analysis was utilized to define the protein expression of epithelial-mesenchymal transition (EMT) markers and NCAPH. Xenograft tumor was established for exploration into the function of circEIF3I in CRC metastasis to the liver and lung. The binding between miR-328-3p and circEIF3I or NCAPH was predicted through ENCORI or TargetScan platform and ascertained through dual-luciferase reporter assays. circEIF3I and NCAPH expression were found to be elevated in CRC tissues and cells, while miR-328-3p was downregulated. Functionally, circEIF3I knockdown inhibited CRC cell migration, invasion, EMT, and tumor metastasis. Mechanistic analyses revealed that circEIF3I can target miR-328-3p, while NCAPH was targeted by miR-328-3p. Furthermore, circEIF3I facilitated NCAPH expression in CRC cells by sequestering miR-328-3p. Notably, miR-328-3p inhibitor or NCAPH overexpression negated the effects of circEIF3I knockdown on preventing CRC progression in vitro. Taken together, circEIF3I elevated NCAPH expression by sponging miR-328-3p, thereby promoting CRC metastasis. These findings suggest that the circEIF3I/miR-328-3p/NCAPH axis represents a novel therapeutic target for CRC.
Primary leiomyosarcoma of the kidney is an extremely rare disease. No single case of primary renal leiomyosarcoma was reported at National Taiwan University Hospital during 1995-2010. Here, we report the case of a 74-...
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Primary leiomyosarcoma of the kidney is an extremely rare disease. No single case of primary renal leiomyosarcoma was reported at National Taiwan University Hospital during 1995-2010. Here, we report the case of a 74-year-old woman who presented with intermittent lower abdominal pain for 2 months. A heterogeneous tumor was found in the left kidney. The clinical presentation suggested renal cell carcinoma. A diagnosis of leiomyosarcoma was made based on the pathological examination. The patient underwent radical nephrectomy. No distant metastasis was noted. No adjuvant chemotherapy or radiotherapy was provided after surgery. Copyright (C) 2012, Taiwan Surgical Association. Published by Elsevier Taiwan LLC. All rights reserved.
Groups of 8 lambs were allocated to one of five concentrate diets supplemented with all-rac-alpha-tocopheryl acetate containing 30 (C30), 60 (C60), 120 (C120), 250 (C250) and 500 (C500) mg/kg dry matter. Two other gro...
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Groups of 8 lambs were allocated to one of five concentrate diets supplemented with all-rac-alpha-tocopheryl acetate containing 30 (C30), 60 (C60), 120 (C120), 250 (C250) and 500 (C500) mg/kg dry matter. Two other groups were fed grass silage and 400 g/day concentrate with 60 (S60) or 500 (5500) mg alpha-tocopheryl acetate/kg dry matter. Within diet, vitamin E level did not affect growth performance or carcass characteristics. Basal diet did not affect final live weight, conformation and fatness scores. M. semimembranosus from S lambs contained more alpha-tocopherol than that of C lambs on the same intake and by day 6 in MAP (75% 02/25%CO2) chroma and a* were below acceptable levels in C30 lambs. TBARS were higher in C30 and C60 muscle than in other treatments (P<0.001) after 3 and 6 days display. Muscle fatty acid composition varied with basal diet but lipid oxidation depended more on vitamin E concentration with an initial concentration of 1.9 mu g/g muscle preventing significant lipid oxidation. (C) 2011 Elsevier Ltd. All rights reserved.
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