Guinea pig thyroid protein with a sedimentation coefficient of 12 S was purified by sucrose gradient centrifugation. Its iodine content is one-tenth that found in thyroglobulin (19 S) obtained from the same preparatio...
Guinea pig thyroid protein with a sedimentation coefficient of 12 S was purified by sucrose gradient centrifugation. Its iodine content is one-tenth that found in thyroglobulin (19 S) obtained from the same preparation. It was shown that this naturally occurring thyroid protein is not derived from and does not dimerize to 19 S thyroglobulin. While it does not interchange with undissociated 19 S, it combines with the 12 S subunits derived from 19 S to form hybrid molecules of thyroglobulin. It is inferred from the hybridization experiments that thyroglobulin consists of 2 different 12 S subunits, 1 of them being equivalent to the naturally occurring 12 S molecule.
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