目的探讨复方地黄颗粒和毛蕊花糖苷治疗帕金森病(P D)的作用靶标。方法采用6-羟基多巴胺(6-OHDA)偏侧损毁黑质制备PD大鼠模型,分层随机分为模型组、美多芭组、毛蕊花糖苷组、复方地黄颗粒(低、中、高剂量)组,另设不作处理的正常对照组和只注射抗坏血酸的假手术组,每组12只。复方地黄颗粒(3.5、7、14g/kg)、毛蕊花糖苷(60mg/kg)和美多芭(150mg/kg)用蒸馏水溶解后分别给予复方地黄颗粒低、中、高剂量组,毛蕊花糖苷组和美多芭组大鼠灌胃,1次/d。正常对照组、假手术组和模型组以等体积蒸馏水(2ml/只)灌胃。灌胃6周后,取大鼠患侧黑质、纹状体,应用RT-PCR法检测各组大鼠N-甲基-D-天冬氨酸受体1(NMDA-R1)、NMDA-R2、γ-氨基丁酸B1受体(GABA-RB1)m RNA水平,HPLC法检测天门冬氨酸(ASP)、谷氨酸(GLU)、甘氨酸(GLY)、γ-氨基丁酸(GABA)等含量变化,免疫组化法检测酪氨酸羟化酶(TH)阳性细胞数。结果复方地黄颗粒和毛蕊花糖苷可使PD模型大鼠右侧纹状体NMDA-R1、NMDA-R2 m RNA水平明显降低,GABA-RB1 m RNA表达升高(P<0.01);ASP、GLU含量升高,GLY、GABA含量降低(P<0.01);TH阳性表达细胞数明显增多(P<0.01)。其中以复方地黄颗粒中剂量组改善最为明显,差异有统计学意义(P<0.01)。镜下观察可见TH阳性信号定位于细胞质,在正常对照组、假手术组的右侧黑质均可见密集的TH阳性细胞,呈棕褐色,细胞形状主要为圆形或卵圆形,少数呈多边形;模型组右侧黑质可见少量、稀疏分布的TH阳性细胞;给药干预后TH阳性细胞数明显增加。结论复方地黄颗粒和毛蕊花糖苷的作用靶标可能包括TH以及氨基酸类神经递质及其受体基因。
Acute lung injury(ALI)/acute respiratory distress syndrome(ARDS),the complicated and devastating illness,resulted from various processes of systemic inflammatory response syndrome(SIRS),injure directly or indirectly t...
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Acute lung injury(ALI)/acute respiratory distress syndrome(ARDS),the complicated and devastating illness,resulted from various processes of systemic inflammatory response syndrome(SIRS),injure directly or indirectly the *** the advance of investigations in SIRS and multiple organs disfunction syndrome(MODS),inflammation development and control have been considered to be the important mechanism of ALI/*** research hotspot is also focused on the inflammatory cells and *** decoction can influence on the functions of inflammatory cells and cytokines,so the strategies of anti-inflammatory treatment on immunoregulation became the key point for prevention and treatment of ALI/ARDS with traditional Chinese medicine.
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