Objective Estrogen may play an important role in visceral pain sensation since chronic visceral pain conditions arc more common in females than in ***(estrogen synthase)the enzyme that catalyzes the transformation of ...
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Objective Estrogen may play an important role in visceral pain sensation since chronic visceral pain conditions arc more common in females than in ***(estrogen synthase)the enzyme that catalyzes the transformation of androgens into estrogens is a member of the P450 superfamily of cytochrome enzymes and a product of the CYP19 *** is well established that the conversion of androgens to estrogens within the central nervous system(CNS)is an essential means by which estrogen regulates many physiological and behavioral processes such as sexual differentiation of the *** rostral ventromedial medulla(RVM)is an essential part of the endogenous pain modulation pathway which project to the spinal cord to facilitate or inhibit nociceptive transmission in the dorsal *** present study was aimed to test the hypothesis that locally synthesized estrogen in RVM may be involved in modulation of visceral pain via activating the G-protein coupled estrogen receptor ***:Immunohistochemical analysis Western blot analysis and behavioral testing of visceral ***:(1)Immunohistochemistry and western blot analysis confirmed the presence of aromatase in ***-expression of aromatase and GPR30 within RVM was confirmed by immunofluorescence assay.(2)The pseudoaffective behavior(abdominal constriction)in response to colorectal distension was enhanced with concomitant increase in phosphorylation of theμ-type opioid receptor(MOR)and decrease in total MOR in RVM in the cyclophosphamide(CPM)-induced chronic cystitis rat model as compared to the control rats indicative of colorectal hyperalgesia.(3)The colorectal hyperalgesia was attenuated by chronic application of the GPR30 antagonist G15 into cerebellomedullary cistern as was the phosphorylation of MOR in RVM of CPM-treated rats.(4)Similarly chronic intracisternal application of the aromatase inhibitors(fadrozole and letrozole)could also attenuate the colorectal hyperalgesia and the phosphorylation of MOR
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