Aims The aim of this study is to investigate the regulatory mechanism of EDAR in cutaneous wound *** At the molecular level,STUB1 was identified as a potential interactor of EDAR through a yeast two-hybrid assay,and t...
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Aims The aim of this study is to investigate the regulatory mechanism of EDAR in cutaneous wound *** At the molecular level,STUB1 was identified as a potential interactor of EDAR through a yeast two-hybrid assay,and the interaction was confirmed by co-immunoprecipitation,immunofluorescence,and GST pull-down *** vitro and in vivo ubiquitination assays,along with a degradation assay,were employed to investigate the role of STUB1 in mediating the ubiquitination and degradation of *** sgRNA for STUB1 was constructed and validated in HaCaT cells,and its impact on cell migration and proliferation was assessed through a scratch assay and cell ***-seq analysis was performed to identify the intracellular responsive genes,and the results were verified by RT-qPCR and *** wound models were established in keratinocytes-specific Edar or Stub1 conditional knockout(cKO) mice to investigate the impact of STUB1 or EDAR on wound *** The Edarfl/flK14cre mice exhibited the ectodermal dysplasia phenotype and demonstrated impaired wound healing on the third day ***1 directly interacts with EDAR,with the interaction occurring primarily within the intracellular domain of EDAR and the N-terminal fragment(1-127) of *** an E3 ubiquitin ligase,STUB1 facilitated the ubiquitination of EDAR at the K252 and K81 sites,subsequently influencing its *** knockout of STUB1 promotes the migration and proliferation of HaCaT *** results of RNA-seq indicated that KRT6 may be a potential responsive molecule regulated by the STUB1-EDAR/NF-κB *** wound healing of Edarfl/flK14cre mice demonstrated that the regulation of STUB1 on epithelial function facilitates murine wound healing during the proliferative phase and promotes the maturation of the *** STUB1 was identified as the first E3 ubiquitin ligase for EDAR,which regulates epithelial proliferation and migration mainly through the
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