Epigenetic information,including histone modifications,DNA methylation,chromatin structure etc.,plays critical roles in regulating the expression of developmental genes during embryo development in ***,by combining hi...
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Epigenetic information,including histone modifications,DNA methylation,chromatin structure etc.,plays critical roles in regulating the expression of developmental genes during embryo development in ***,by combining high-throughput epigenomics technology and bioinformatics analysis,we revealed the features of establishing key histone modifications[1]and nucleosome organization[2]during the onset of zygotic transcription in ***,we extended our previous studies to the establishment of chromatin accessibility,another important epigenetic information,during the early embryogenesis of ***,we presented genome-wide map of the histone-modification landscape of mouse pre-implantation embryos during zygotic genome activation and the first cell lineage differentiation[3].Consistent with the findings in zebrafish,zygotic H3K4me3 accumulated more rapidly than H3K27me3 in ***,H3K4me3 and H3K27me3 possess distinct features of sequence preference and dynamics in pre-implantation ***,the breadth of the H3K4me3 domain is a highly dynamic *** broad H3K4me3 domain (wider than 5 kb) is associated with higher transcription activity and cell identity in pre-implantation *** also found that the bivalency (i.e.,co-occurrence of H3K4me3 and H3K27me3) in early embryos is relatively infrequent and *** together,our studies in zebrafish and mouse facilitated further exploration of the mechanism for epigenetic regulation in early embryos.
该文旨在探讨基于冰冻切片样品进行苏木素–伊红(hematoxylin-eosin,H&E)染色和快蓝(luxol fast blue,LFB)染色方法的优化及其在实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)组织病理分析中的应用。...
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该文旨在探讨基于冰冻切片样品进行苏木素–伊红(hematoxylin-eosin,H&E)染色和快蓝(luxol fast blue,LFB)染色方法的优化及其在实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)组织病理分析中的应用。取正常及EAE建模后的C57BL/6小鼠脊髓样品各5份,每份脊髓样品一分为二,分别制成冰冻切片和石蜡切片样品,通过设置不同优化条件对这两组切片进行H&E和LFB染色,比较两组切片染色的结果。石蜡切片经过H&E染色和LFB染色后细胞的形态结构和组织结构清晰,冰冻切片H&E染色和LFB染色后细胞结构清晰度几乎和石蜡切片一致,进一步将该方法应用于分析β-arrestin2基因敲除对于小鼠EAE发生中的病理变化,发现能够很好地重复出基因敲除加重EAE疾病这一现象。冰冻切片染色优化后,基于冰冻切片进行H&E染色和LFB染色可以达到石蜡切片类似的效果,并且实验过程简洁快速,大大缩短了实验时间,提高了效率,可以较好地应用于分析自身免疫疾病病理变化,该优化方法将在自身免疫疾病特别是多发性硬化动物模型的研究中有广泛的应用。
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