Successful crossbreeding between Oreochromis niloticus and Sarotherodon melanotheron to produce a commercial hybrid has been *** karyotypes and isoenzyme of these two species and their reciprocal hybrids（*** ♀ ×...
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Successful crossbreeding between Oreochromis niloticus and Sarotherodon melanotheron to produce a commercial hybrid has been *** karyotypes and isoenzyme of these two species and their reciprocal hybrids（*** ♀ × *** ♂,*** ♀ × *** ♂,the last not included in the isoenzyme study） were investigated via metaphase chromosomes obtained from head kidney cells and electropherogram of lactate dehydrogenase（LDH） isoenzymes from the liver,kidney,white muscle,heart,and eye *** diploid chromosome number（2n=44） and the fundamental number（NF=50） of the four tilapia genotypes were the ***,the karyotype of *** had three pairs of sub-metacentric（sm）,twelve pairs of sub-telocentric（st）,and seven pairs of telocentric（t） chromosomes,while *** had one pair of metacentric（m）,two pairs of sm,12 pairs of st,and seven pairs of t *** reciprocal hybrids both showed a mixed karyotype range between their parents：0.5 pair of m,2.5 pairs of sm,12 pairs of st,and seven pairs of t *** view of the electropherogram of isozymes,only the LDH of the kidney showed significant clear bands,with five bands in ***,three bands in ***,and duplicated six bands in the *** bands varied depending on their activities and *** considered that the differences in karyotype and isoenzyme were related to the genetic mechanism for post-mating isolation,and provided some additional basic genetic background of their taxonomy.
本研究应用18个微卫星分子标记,对白斑狗鱼(Esox lucius L.)3个中国新疆群体(乌伦古湖、吉力湖和6号湖)和1个匈牙利巴拉顿湖群体的遗传结构进行了分析。结果表明,3个中国白斑狗鱼群体的平均等位基因丰富度(AR)、平均观测杂合度(HO)和平...
详细信息
本研究应用18个微卫星分子标记,对白斑狗鱼(Esox lucius L.)3个中国新疆群体(乌伦古湖、吉力湖和6号湖)和1个匈牙利巴拉顿湖群体的遗传结构进行了分析。结果表明,3个中国白斑狗鱼群体的平均等位基因丰富度(AR)、平均观测杂合度(HO)和平均期望杂合度(HE)均显著低于匈牙利巴拉顿湖群体(P<0.05),而匈牙利群体的平均近交系数(FIS)高于中国群体;经SMM和TPM模型检测,匈牙利白斑狗鱼群体存在显著的遗传瓶颈信号(P<0.001);AMOVA和群体两两比较的FST值表明,中国与匈牙利白斑狗鱼的遗传分化十分显著(P<0.01);NJ树、主成分分析(PCA)进一步证实中国白斑狗鱼与匈牙利白斑狗鱼群体间存在显著的遗传差异和分化。此外,贝叶斯遗传聚类结果表明,中国新疆6号湖白斑狗鱼群体极可能来源于乌伦古湖,而非吉力湖。
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