Background:Ovarian cancer(OC) is the most lethal gynecologic tumor characterized by high-rate *** in accurately delineating the metastatic pattern have greatly restricted the improvement of treatment in OC *** studies...
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Background:Ovarian cancer(OC) is the most lethal gynecologic tumor characterized by high-rate *** in accurately delineating the metastatic pattern have greatly restricted the improvement of treatment in OC *** studies have leveraged mitochondrial DNA(mtDNA) mutations as efficient lineage-tracing markers of tumor ***:We applied multi-region sampling and high-depth mtDNA sequencing to portray the metastatic patterns in advanced-stage OC *** mtDNA mutations were profiled from a total of 149 primary and 246 metastatic tumor tissue samples in 35 OC ***:Our results revealed remarkable sample-level and patient-level *** addition,distinct mtDNA mutational patterns were observed between primary and metastatic OC *** analysis identified the different mutational spectra between shared and private mutations among primary and metastatic OC *** of clonality index calculated based on mtDNA mutations supported monoclonal tumor origin in 15 of 17patients with bilateral ovarian ***,mtDNA-based spatial phylogenetic analysis revealed distinct patterns of OC metastasis,in which linear metastatic pattern exhibited low degree of mtDNA mutation heterogeneity and short evolutionary distance,whereas parallel metastatic pattern showed the opposite ***,a mtDNA-based tumor evolutionary score(MTES) related to different metastatic patterns was *** data showed that patients with different MTES responded differently to the combined debulking surgery and ***,we observed that tumor-derived mtDNA mutations were more likely to be detected in ascitic fluid than plasma samples.
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