Adenovirus vectors have become a very popular tool for gene transfer into mammalian ***, the construction of first generation adenovirus vectors was a time-consumingprocess, requiring 2 to 3 months of intensive labor....
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Adenovirus vectors have become a very popular tool for gene transfer into mammalian ***, the construction of first generation adenovirus vectors was a time-consumingprocess, requiring 2 to 3 months of intensive labor. The AdEasy system simplifies the productionof such viruses. The adenovirus E3 region encodes products that counteract host defensemechanisms, which are not essential for viral replication in vitro, and therefore the E3 region wasdeleted in backbone vectors of AdEasy system to give more space for insertion of foreign ***, it was suggested that construction of Ad vectors to include E3 expression cassetteswould improve efficacy and safety of adenoviral-based gene therapy protocols (Cancer Res. 59:4200). We generated an adenoviral backbone vector AdEasy-E3, which contains full length of
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