AIM:To study immunoinhibitory effects and preliminary mechanism of IL-10 and trichosanthin.METHODS:Surface molecule expression on antigen processing cells (APC) was stained with fluorescence and analyzed by FACScan. B...
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AIM:To study immunoinhibitory effects and preliminary mechanism of IL-10 and trichosanthin.
METHODS:Surface molecule expression on antigen processing cells (APC) was stained with fluorescence and analyzed by FACScan. B7-1 mRNA expression was detected with nested RT-PCR.
RESULTS:IL-10 2 mg.L-1 and trichosanthin 10 mg.L-1 inhibited B7-1 molecule expression. By contrast, they had not the same effects on ICAM-1. IL-10 and trichosanthin down-regulated LFA-1 expression, but had no regulatory effect on CD40. IL-10 and trichosanthin dramatically inhibited T-cell proliferation and IL-2 production. B7-1 mRNA expression was undetectable in APC treated with IL-10 and trichosanthin.
CONCLUSION:IL-10 and trichosanthin inhibit surface molecule expression on APC. They exert multiple immunoinhibitory effects.
Objective To study the role of placental leptin in intrauterine cord leptin production and its relationship with neonatal anthropometry. Methods Forty women and their babies (40) were enrolled in this study. Placent...
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Objective To study the role of placental leptin in intrauterine cord leptin production and its relationship with neonatal anthropometry. Methods Forty women and their babies (40) were enrolled in this study. Placental tissues were assayed for leptin mRNA by reverse transcription/polymerase chain reaction (RT/PCR), and assayed for the obese gene protein leptin by Western-blot and immunohistochemistry. Blood was taken from the umbilical cord of the babies at delivery. Serum leptin was measured by radio-immunoassay. Neonatal anthropometric measurements were recorded within 48 hours after delivery. Linear regression analysis was used to explore the relationship between placental leptin, cord leptin and neonatal anthropometric measures. Results The obese gene was expressed in placental tissue at comparable or greater levels than that in adipose tissue. The placentas of the small for gestational age (SGA) neonates expressed leptin mRNA and protein at significantly lower levels than those of the appropriate for gestational age (AGA) neonates (P=0.0034 and 0.0076), while the placentas of the large for gestational age (LGA) neonates expressed leptin mRNA and protein at significantly higher levels than those of the AGA neonates (P=0.043 and 0.021). Linear regression analysis showed placental ob gene transcription and leptin translation correlated significantly with cord leptin (r=0.39 and 0.43), and neonatal Ponderal Index (r=0.66 and 0.69). Conclusion The placenta provides a source of leptin for the growing fetus, and this placental leptin might be a growth factor in intrauterine fetal development.
Objective To express mouse peroxisome proliferator activated receptor γ2 (mPPARγ2) in NIH3T3 fibroblasts mediated by the recombinant retrovirus and study its *** The mPPARγ2 gene was subcloned into retrovirus vec...
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Objective To express mouse peroxisome proliferator activated receptor γ2 (mPPARγ2) in NIH3T3 fibroblasts mediated by the recombinant retrovirus and study its *** The mPPARγ2 gene was subcloned into retrovirus vector pGCEN to generate the recombinant pGCEN/mPPARγ2. Then it was packaged into PA317 cells and selected with G418. Viral supernatants were harvested and then used to infect NIH3T3 fibroblasts. PPARγ activator 5,8,11,14-eicosatetraynoic acid (ETYA) was used to induce the mPPARγ2-expressing NIH3T3 cells into adipocyte *** The recombinant retrovirus pGCEN/mPPARγ2 was constructed, and the higher titers of the viral supernatants were obtained. mPPARγ2 was expressed in NIH3T3 cells mediated by the recombinant retrovirus. Lipid accumulation obviously existed in these induced adipocytes which morphologically resembled mature adipocytes in vivo and expressed tissue specific adipocyte P2 (AP2) and Leptin *** An adipocyte differentiation model in vitro was successfully established. The work is the basis for further research on the molecular mechanism of adipocyte differentiation induced by PPARγ2.
odeling · mechanismObjective To investigate mechanisms of anti hypertension and anti cardiovascular remodeling by phenylalanine (phe) in spontaneously hypertensive rats (SHRs) Methods The comparison of ...
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odeling · mechanismObjective To investigate mechanisms of anti hypertension and anti cardiovascular remodeling by phenylalanine (phe) in spontaneously hypertensive rats (SHRs) Methods The comparison of blood pressure (BP) increment with the ages and cardiovascular changes of SHRs was made between the 3% phe intervented group (SHR phe) and the control SHRs group Detection of the structural changes with the VIDAS digital vedio frequency processing technique and light and electron microscopy were made The cell growth and proliferation of cultured smooth muscle cells (CSMCs) of the thoracic aortas or myocardial fibroblasts were evaluated by measuring the 3 H thymidine counts per minute (cpm) incorporated into the new synthesized desoxyribonucleic acid (DNA) and determining the cell number with the crystal violet stain technique The Ca 2+ influx was measured in counts/min of 45 CaCl 2 after incubating it with 5 different concentrations of phenylalanine and the intracellular [Ca 2+ ] i by Fura Ⅱ/Am indicator The total messenger ribonucleic acid (mRNA) of the myocardium was extracted and Northern blot analysis was performed with the probe collagen α 2(Ⅰ)cDNA The tyrosine hydroxylase (TH) activity was measured by high performance liquid chromatography (HPLC) with electrochemical detector after having reacted with its substrate tyrosine and other reagents The catecholamine contents in brain homogenat were detected by HPLC method The comparison of pharmacokinetics of phenylalanine among SHR phe, SHRs and control Wistar Kyoto (WKY) rats was made after intravenous injection of 3 H L phe (1?ml/kg) by PK GRAPH Program for kinetic calculation The 3H L phe uptake by CSMCs after incubating for difinite intervals was also detected and compared Results Phenylalanine could prevent the increase of BP with ages and the heart weight (heart/body weight index) The aortic media thickness and the collagen content in
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