In previous study we reported that pretreatment with plasmolysis enhanced somatic embryo formation in hypocotyls of Eleutherococcus *** the present study,the expression level of callose synthase gene in embryos of ***...
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In previous study we reported that pretreatment with plasmolysis enhanced somatic embryo formation in hypocotyls of Eleutherococcus *** the present study,the expression level of callose synthase gene in embryos of *** in response to 2,4-D,sucrose and mannitol treatments was analyzed by *** results show that plasmolysis pretreatment using sucrose and mannitol significantly promoted the expression of callose synthase ***,the thicker cell walls of explant plasmolyzed compared with controls were observed during the somatic *** suggest that the callose may make the cells in epidermis separate from neighboring cells and then develop into embryogenic potential cells.
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