Based on the genetic inheritance and segregation of random amplified polymorphism DNA(RAPDs) markers,the analysis of linkage groups for birch was performed by using a pseudo-testcross strategy.A segregating population...
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Based on the genetic inheritance and segregation of random amplified polymorphism DNA(RAPDs) markers,the analysis of linkage groups for birch was performed by using a pseudo-testcross strategy.A segregating population comprising of 79 progenies from the cross between Betula pendula and *** was obtained.A set of 1 000 random oligonucleotide primers were screened,and 178 primers were selected to generate RAPD markers within a sample of 79 F\-1 progenies.A total of 296 segregating sites was *** them,273 sites belonged to 1∶1 segregating site,and 36 sites were found distorted from the 1∶1 *** 273 sites segregating 1∶1(testcross configuration) were used to construct parent-specific linkage group,among which 137 sites were found for *** and 136 sites for *** resulting linkage maps consisted of 117 marker sites in 12 groups(four or more sites per group),2 triples and 3 pairs for ***,which covered the map distance about 1 533.3 cM(Kosambi units),and the average map distance between adjacent markers was 16.4 *** the ***,162 linked marker sites were integrated into 12 groups(four or more sites per group),2 triples and 4 pairs,which covered the map distance about 1 847.8 cM,and the average map distance between adjacent markers was 19.2 *** calculated genome coverage rate of *** and *** were respectively 68.5% and 68.9%.Further study is required to integrate the two maps to one density map and to locate important genes on the maps.
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