Based on the genetic inheritance and segregation of random amplified polymorphism DNA(RAPDs) markers,the analysis of linkage groups for birch was performed by using a pseudo-testcross strategy.A segregating population...
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Based on the genetic inheritance and segregation of random amplified polymorphism DNA(RAPDs) markers,the analysis of linkage groups for birch was performed by using a pseudo-testcross strategy.A segregating population comprising of 79 progenies from the cross between Betula pendula and *** was obtained.A set of 1 000 random oligonucleotide primers were screened,and 178 primers were selected to generate RAPD markers within a sample of 79 F\-1 progenies.A total of 296 segregating sites was *** them,273 sites belonged to 1∶1 segregating site,and 36 sites were found distorted from the 1∶1 *** 273 sites segregating 1∶1(testcross configuration) were used to construct parent-specific linkage group,among which 137 sites were found for *** and 136 sites for *** resulting linkage maps consisted of 117 marker sites in 12 groups(four or more sites per group),2 triples and 3 pairs for ***,which covered the map distance about 1 533.3 cM(Kosambi units),and the average map distance between adjacent markers was 16.4 *** the ***,162 linked marker sites were integrated into 12 groups(four or more sites per group),2 triples and 4 pairs,which covered the map distance about 1 847.8 cM,and the average map distance between adjacent markers was 19.2 *** calculated genome coverage rate of *** and *** were respectively 68.5% and 68.9%.Further study is required to integrate the two maps to one density map and to locate important genes on the maps.
In previous study we reported that pretreatment with plasmolysis enhanced somatic embryo formation in hypocotyls of Eleutherococcus *** the present study,the expression level of callose synthase gene in embryos of ***...
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In previous study we reported that pretreatment with plasmolysis enhanced somatic embryo formation in hypocotyls of Eleutherococcus *** the present study,the expression level of callose synthase gene in embryos of *** in response to 2,4-D,sucrose and mannitol treatments was analyzed by *** results show that plasmolysis pretreatment using sucrose and mannitol significantly promoted the expression of callose synthase ***,the thicker cell walls of explant plasmolyzed compared with controls were observed during the somatic *** suggest that the callose may make the cells in epidermis separate from neighboring cells and then develop into embryogenic potential cells.
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