PtDr101基因从三倍体毛白杨(Popu1us tomentosa× P. bolleana)×***]中克隆获得,能够编码NBS-LRR型蛋白,受到水杨酸和甲基茉莉酸诱导表达,是一种广谱的抗病基因。本研究利用实时定量PCR技术分析转PtDr101基因hpRNA干扰载体的...
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PtDr101基因从三倍体毛白杨(Popu1us tomentosa× P. bolleana)×***]中克隆获得,能够编码NBS-LRR型蛋白,受到水杨酸和甲基茉莉酸诱导表达,是一种广谱的抗病基因。本研究利用实时定量PCR技术分析转PtDr101基因hpRNA干扰载体的表达模式,分析发现4个表达模式显著差异的转基因株系,为进一步功能鉴定提供材料。本研究表明实时定量PCR能够高灵敏度、精确地检测hpRNA干扰情况下的基因表达量。
[Objective] The aim of this study was to perform genome-wide analysis of glucose-6-phosphate dehydrogenase（G6PDH） and reveal its evolution in Eucalyptus grandsis.[Method] The gene character,protein sequence and phyl...
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[Objective] The aim of this study was to perform genome-wide analysis of glucose-6-phosphate dehydrogenase（G6PDH） and reveal its evolution in Eucalyptus grandsis.[Method] The gene character,protein sequence and phylogenetic tree of G6PDH gene were analyzed by BLAST and other bioinformatics software within Eucalyptus grandsis whole genome database.[Result] Six G6PDH genes,including one cytomic type and five plastids,were detected in the *** *** the G6PDHs have conserved motifs of motif 1,motif 2,motif 3,motif 7,motif 9 and motif ***,promoter sequences of all *** G6PDH contain TATA box,enhancer,light-responsive,hormone-responsive and stress-responsive regulatory elements.[Conclusion] This study provided reference for the further revealing molecular function of *** G6PDH gene family
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