The fiber length trait （FLT） of 538 individuals from nature birch population in Maorshan region, Heilongjang, China were measured, of which 100 individuals were selected as representative variety of correlated fragm...
详细信息
The fiber length trait （FLT） of 538 individuals from nature birch population in Maorshan region, Heilongjang, China were measured, of which 100 individuals were selected as representative variety of correlated fragments screening with random amplified polymorphism DNA （RAPD） technique. In total of 20 RAPD primers were tested through multiple regression analysis between amplified strip and the character behaviors, and a correlative segment BFLR-16 was obtained. The correlation coefficient between BFLI-16 and FLT was 0.6144, with the significant level of 1%. BFLI-16 was then cloned, sequenced and transformed into SCAR marker. The percentage of identifying long fiber birches by this SCAR was more than 92. The result indicates that the SCAR markers has high specificity for the long fiber individuals and is highly linked with the gene controlling the character of fiber length, and its existence is significantly correlative with the increase in the fiber length.
Based on the genetic inheritance and segregation of random amplified polymorphism DNA(RAPDs) markers,the analysis of linkage groups for birch was performed by using a pseudo-testcross strategy.A segregating population...
详细信息
Based on the genetic inheritance and segregation of random amplified polymorphism DNA(RAPDs) markers,the analysis of linkage groups for birch was performed by using a pseudo-testcross strategy.A segregating population comprising of 79 progenies from the cross between Betula pendula and *** was obtained.A set of 1 000 random oligonucleotide primers were screened,and 178 primers were selected to generate RAPD markers within a sample of 79 F\-1 progenies.A total of 296 segregating sites was *** them,273 sites belonged to 1∶1 segregating site,and 36 sites were found distorted from the 1∶1 *** 273 sites segregating 1∶1(testcross configuration) were used to construct parent-specific linkage group,among which 137 sites were found for *** and 136 sites for *** resulting linkage maps consisted of 117 marker sites in 12 groups(four or more sites per group),2 triples and 3 pairs for ***,which covered the map distance about 1 533.3 cM(Kosambi units),and the average map distance between adjacent markers was 16.4 *** the ***,162 linked marker sites were integrated into 12 groups(four or more sites per group),2 triples and 4 pairs,which covered the map distance about 1 847.8 cM,and the average map distance between adjacent markers was 19.2 *** calculated genome coverage rate of *** and *** were respectively 68.5% and 68.9%.Further study is required to integrate the two maps to one density map and to locate important genes on the maps.
暂无评论