S-RNase-mediated gametophytic self-incompatibility （GSI） is controlled by a multiallelic S-locus at which two separate genes, the female （pistil） and male （pollen） specificity determinants, are tightly linked. T...
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S-RNase-mediated gametophytic self-incompatibility （GSI） is controlled by a multiallelic S-locus at which two separate genes, the female （pistil） and male （pollen） specificity determinants, are tightly linked. This review described both the identification of pollen specific F-box genes, SLF/SFBs, in Antirrhinum, Petunia and Prunus species and the demonstration of SLF/SFB as pollen determinant together with their functions in GSI response. Recent studies of how the pollen determinant functions in pollination reaction revealed that pollen determinant interacted with S-RNases in a non-allele-specific manner. It targeted all of the non-self S-RNases for ubiquitination through a functional SCF complex and subsequent degradation via 26S proteasome pathway in compatible reaction. It allows pollen tube to reach into the embryo sac and to finish double fertilization. In incompatible response, the intact self S-RNases were left to function as a cytotoxin that degrades self-pollen tube RNA, resulting in the cessation of pollen tube growth.
研究了蒙花1、2号金银花带芽茎段的丛芽诱导和快速繁殖技术.结果表明:初代培养以M S为基本培养基,附加6-BA1.0 m g.L-1+NAA 0.2 m g.L-1诱导腋芽能得到理想的效果;在继代培养中,通过6-BA和NAA的组合试验,④号M S+BA 1.5m g.L-1+NAA 0.02...
详细信息
研究了蒙花1、2号金银花带芽茎段的丛芽诱导和快速繁殖技术.结果表明:初代培养以M S为基本培养基,附加6-BA1.0 m g.L-1+NAA 0.2 m g.L-1诱导腋芽能得到理想的效果;在继代培养中,通过6-BA和NAA的组合试验,④号M S+BA 1.5m g.L-1+NAA 0.02 m g.L-1和⑥号M S+BA 1.5 m g.L-1+NAA 0.05 m g.L-1及⑧号M S+BA 2.0 m g.L-1+NAA0.05 m g.L-1的诱导丛芽效果都较好;将无菌苗置于(1/2)M S+NAA 1.0 m g.L-1的培养基诱导生根,诱导率达92%.
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