Objective To determine whether learning deficits could be seen in transgenic mice expressing human amyloid precursor protein 770 (APP 770 ) Methods Female heterozygous transgenic and nontransgenic mice aged 3,...
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Objective To determine whether learning deficits could be seen in transgenic mice expressing human amyloid precursor protein 770 (APP 770 ) Methods Female heterozygous transgenic and nontransgenic mice aged 3, 6 and 9 months at the start of testing were used, with eight mice in each age group All mice were subjected to various behavioral tasks including the Y maze task and the Morris water maze After behavioral testing, the mice were sacrificed, and their brain tissues were used for measuring the choline acetyltransferase (ChAT) activity Results Nine month old transgenic mice exhibited spatial learning deficits in the Morris water maze and in spontaneous alternation in the Y maze, compared with those of the age matched non transgenic mice The behavioral changes accompanied a reduction of ChAT activity in the cortical and hippocampal regions of transgenic mice On the other hand, these behavioral deficits were not observed in transgenic mice either at 3 or at 6 months of age, in which ChAT activity remained unchanged Conclusions The present results show that the learning impairment observed in 9 month old APP 770 transgenic mice are accompanied by a decrease in cortical and hippocampal ChAT activities This suggests that cholinergic deficits may be involved in the learning impairment observed in these APP 770 mice This model will be a useful tool in advancing our understanding of the relationship between the cholinergic system and the cognitive deficits observed in Alzheimer's disease (AD)
为了解梨黑星病抗性遗传规律并为梨黑星病抗性育种早期选择提供理论依据,以新苹梨(梨黑星病抗性品种)×雪花梨(梨黑星病易感品种)的杂交后代为试材,进行了田间自然感病和棚内接种梨黑星病(Venturia nashicolaTanak et Yamamota)病...
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为了解梨黑星病抗性遗传规律并为梨黑星病抗性育种早期选择提供理论依据,以新苹梨(梨黑星病抗性品种)×雪花梨(梨黑星病易感品种)的杂交后代为试材,进行了田间自然感病和棚内接种梨黑星病(Venturia nashicolaTanak et Yamamota)病菌后的抗性调查,并进行了AFLP分子标记的研究。结果表明,棚内接种梨黑星病菌后,杂交后代抗感分离规律符合双假测交的遗传规律;研究建立的AFLP分子标记体系可以用于梨黑星病的抗性基因标记,并初步找到了与梨黑星病抗性基因相关的分子标记。
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