[Objective] The research aimed to induce the expression of FMDV structural protein VP1 in *** and purify the protein,then detect the activity.[Method] The fragment coding VP1 was amplified by PCR and doubly digested w...
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[Objective] The research aimed to induce the expression of FMDV structural protein VP1 in *** and purify the protein,then detect the activity.[Method] The fragment coding VP1 was amplified by PCR and doubly digested with BamH Ⅰ and XhoⅠ,then cloned into expression vector pGEX-4T-1 and pPROExHTb respectively to get recombinant plasmid pGEX-4T-1-VP1 and *** recombinant plasmid pGEX-4T-1-VP1 and pPROExHTb-VP1 was transformed into *** BL21(DE3)and induced by IPTG,fusion protein was identifie...
[Objective] The aim of this study was to identify swine diseases caused by CSFV,PRRSV and PCV2 and thus to analyze its pathogeny chracteristics.[Method] The tissues and viscera of the diseased swine were collected fro...
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[Objective] The aim of this study was to identify swine diseases caused by CSFV,PRRSV and PCV2 and thus to analyze its pathogeny chracteristics.[Method] The tissues and viscera of the diseased swine were collected from Xiangtan of Hunan(Code of HN/XT)to extract DNA and RNA for PCR amplification and ***,the virulent strains were isolated and identified by cell separation technology.[Result] The sequencing analysis results showed that the amino acid homology between CSFV,PRRSV,PCV2 and sequen...
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