[Objective] The study aimed to clone RPO30 gene from Sheeppox virus （SPPV） and predict the structure and function of the sequence. [Method] RPO30 gene of SPPV was cloned with PCR, linked into pMD18-T simple vector a...
详细信息
[Objective] The study aimed to clone RPO30 gene from Sheeppox virus （SPPV） and predict the structure and function of the sequence. [Method] RPO30 gene of SPPV was cloned with PCR, linked into pMD18-T simple vector and then transformed into E. coli DH5a. In blue-white screen, the white colonies were selected to prepare plasmids. The positive plasmids were selected by double digestion and PCR, and then sequenced. Finally, the structure and function of the sequence obtained were predicted by bioinformatics methods. [Results] The RPO30 gene was successfully obtained; its ORF was 585 bp, encoding 193 amino acids and containing a recognition site for Hind III. Moreover, the SPPV RPO30 gene shared different homologies with the RPO30 gene sequences of other pox virus strains from GenBank database. Further analysis by biological software showed that in RPO30 protein, amino acids 4-12, 18-26, 50- 61, 68- 92 and 176-190 had a high possibility to form the active center, and acting to these regions was likely to inactivate the enzyme encoded by the sequence, thus to inhibit viral replication efficiently. [Conclusion] This study will lay foundation for further study on the structure and function of RPO30.
Accompanying with the increasingly saturated genome figures,DNA chip has been widely *** to its advantages of integration,miniaturization and automation,DNA chip becomes a powerful research tool in various research fi...
详细信息
Accompanying with the increasingly saturated genome figures,DNA chip has been widely *** to its advantages of integration,miniaturization and automation,DNA chip becomes a powerful research tool in various research fields including biology,medicine and *** article overviews the application of DNA chip technology in animal medicine from gene expression spectrum research,pathogenic microbial detection,bacterial typing,genetic mutations and polymorphism detection,pathogenic microbial genomics research,as well as its principle and classification.
[Objective] The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the *** varieties suitable for the foreign gene expression....
详细信息
[Objective] The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the *** varieties suitable for the foreign gene expression.[Method] The recombinant *** baculovirus rBmNPV（P1-2A3C） are injected into pupae of original species and hybrids of *** ***,the expression of antigen is detected by ELISA method and compared.[Result] The expression level of P1-2A3C significantly differed in different *** varieties,in which,the hybrids of Qiufeng×TQ78 and Qiufeng×Sijiaojian could be considered as the effective bioreactors for high-level expression of foreign genes.[Conclusion] This study provided the basis for breeding special *** varieties which can highly express target protein of Asia I FMDV.
暂无评论