[Objective] The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the *** varieties suitable for the foreign gene expression....
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[Objective] The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the *** varieties suitable for the foreign gene expression.[Method] The recombinant *** baculovirus rBmNPV(P1-2A3C) are injected into pupae of original species and hybrids of *** ***,the expression of antigen is detected by ELISA method and compared.[Result] The expression level of P1-2A3C significantly differed in different *** varieties,in which,the hybrids of Qiufeng×TQ78 and Qiufeng×Sijiaojian could be considered as the effective bioreactors for high-level expression of foreign genes.[Conclusion] This study provided the basis for breeding special *** varieties which can highly express target protein of Asia I FMDV.
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