[Objective] The research aimed to induce the expression of FMDV structural protein VP1 in *** and purify the protein,then detect the activity.[Method] The fragment coding VP1 was amplified by PCR and doubly digested w...
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[Objective] The research aimed to induce the expression of FMDV structural protein VP1 in *** and purify the protein,then detect the activity.[Method] The fragment coding VP1 was amplified by PCR and doubly digested with BamH Ⅰ and XhoⅠ,then cloned into expression vector pGEX-4T-1 and pPROExHTb respectively to get recombinant plasmid pGEX-4T-1-VP1 and *** recombinant plasmid pGEX-4T-1-VP1 and pPROExHTb-VP1 was transformed into *** BL21(DE3)and induced by IPTG,fusion protein was identifie...
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