Equine infectious anemia virus (EIAV) causes equine infectious anemia and persistent infection as well as repeated high-titer viremia in *** long-terminal repeat (LTR) of EIAV proviral genome contains an eukaryotic pr...
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Equine infectious anemia virus (EIAV) causes equine infectious anemia and persistent infection as well as repeated high-titer viremia in *** long-terminal repeat (LTR) of EIAV proviral genome contains an eukaryotic promoter which plays an important regulation role on transcription and replication of the *** the study,the LTRs of an infectious molecular clone derived from EIAV donkey leukocyte-attenuated vaccine virus (DLA) were substituted with those of EIAV L strain,a virulent EIAV from which DLA strain was derived,resulting in a chimeric plasmid pOK-LTR DLA/*** purified pOK-LTR DLA/L was used to transfect leukocyte cultures from EIAV-negative *** transcriptase (RT) activity was detectable by the 7th passage in cell cultures of the transfection *** cytopathogenic effects typical for EIAV infection were developed since the 8th passage of cell cultures,and virions with cone-shaped core could be observed under electron *** proved that the constructed chimeric pOK-LTR DLA/L is infective and it may be used in future for exploring of molecular mechanism of pathogenesis and immunity of EIAV.
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