为了探究禽网状内皮组织增殖病的快速检测方法,根据禽网状内皮组织增殖病病毒(REV)LTR和gag基因的保守区域各设计并合成一对引物,建立检测插入感染与全病毒感染的SYBR Green I模式的实时荧光PCR方法(Real-time PCR)。以pMD-LTR,pMD-gag...
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为了探究禽网状内皮组织增殖病的快速检测方法,根据禽网状内皮组织增殖病病毒(REV)LTR和gag基因的保守区域各设计并合成一对引物,建立检测插入感染与全病毒感染的SYBR Green I模式的实时荧光PCR方法(Real-time PCR)。以pMD-LTR,pMD-gag重组质粒为标准品建立标准曲线,并对该方法的特异性、敏感性、重复性进行评价。结果表明:该方法线性关系良好;敏感性能检测到10拷贝标准品;能特异性检测REV样品,不与其它禽相关病原发生交叉反应;板内、板间重复试验的变异系数均低于3%。应用该方法对人工感染REV的鸡的心、肝、脾、肺、肾、盲肠扁桃体、腺胃和法氏囊进行初步检测,结果显示法式囊中的含量最高。
[Objective] Infectious bursal disease （IBD） is a highly contagious immuno- suppressive disease caused by infectious bursal disease virus （IBDV）. IBDV is ge- netically prone to mutation, which results in challenges...
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[Objective] Infectious bursal disease （IBD） is a highly contagious immuno- suppressive disease caused by infectious bursal disease virus （IBDV）. IBDV is ge- netically prone to mutation, which results in challenges to the disease prevention and control. Thus, it is necessary to continuously monitor the prevalence of IBDV. [Method] 36 IBDVs were identified from ten provinces in China from 2009 to 2012. Partial fragments of VP2, including the hypervariable region （HVR）, from new iso- lates were sequenced and analyzed through comparisons with published sequences of IBDV, including 18 strains isolated previously by our lab and 24 reference strains from China and around the world. [Result] Phylogenetic analysis showed a co-exis- tence of IBDV strains belonging to classic, variant, attenuated, and very virulent IB- DV （wlBDV） in China. wlBDVs remain the predominant strains in China and the new subgroup was emerging. Alignment analysis revealed several distinct amino acid mutations that might be involved in virulence or antigenicity variation. [Conclu- sion] The results offered evolutionary clues showing the emerging trend of obvious variations and diversity of IBDV in major poultry-producing regions of China particu- larly in recent years. These findings will contribute to a better understanding of the genetic evolution of IBDV.
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