The spike gene(S)of Infectious bronchitis virus(IBV)CK/CH/LDL97Ⅰ/97 isolate was amplified,cloned,sequenced and *** showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino *** precursor of the S protei...
详细信息
The spike gene(S)of Infectious bronchitis virus(IBV)CK/CH/LDL97Ⅰ/97 isolate was amplified,cloned,sequenced and *** showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino *** precursor of the S protein was cleaved into S1 and S2 fragments,which comprised 541 and 626 amino acid residues,*** cleavage site sequence was *** homologies of nucleotide and amino acid sequences of S1 gene between CK/CH/LDL97 Ⅰ/97 and 27 reference IBV strains ranged from 60.6% to 99.6%,and 50.5% to 99.1%,*** CK/CH/LDL97Ⅰ/97 S1 was most similar to that of three proventriculus IBV isolates,Q1(99.1%),J2(98.9%),T3(98.9%),which were isolated in *** analysis of S1 proteins indicated that CK/CH/LDL97Ⅰ/97,Q1,J2 and T3 formed a separated cluster,which might belong to a novel genotype of IBV.
暂无评论