Pear scab caused by Venturia nashicola is one of the most destructive diseases of *** markers linked to scab resistance gene is expected to be useful for improving *** this study,the F1 population derived from the cro...
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Pear scab caused by Venturia nashicola is one of the most destructive diseases of *** markers linked to scab resistance gene is expected to be useful for improving *** this study,the F1 population derived from the cross of ’Huangguan’ and ’Yali’ was analyzed *** resistance of pear to scab was proved to be controlled by a single gene in a dominant *** segregant analysis(BSA) was conducted to screen 64 fluorescent AFLP primer pairs.A marker designated as D3-365 was found to be linked to the resistant *** genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.
研究利用在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR(simple sequence repeat with tailed primer M13)对苹果种质资源的26份材料进行了SSR标记的鉴定,5对引物共获得52个等位基因,平均10.4个...
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研究利用在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR(simple sequence repeat with tailed primer M13)对苹果种质资源的26份材料进行了SSR标记的鉴定,5对引物共获得52个等位基因,平均10.4个。引物对CH04h02可区分全部的供试品种,5对引物多态性好。26份材料5个SSR位点的遗传多样性、多态性信息含量和位点杂合度的变化为0.6620～0.9149、0.6326～0.9086和0.6538～1.0000。这种方法具有经济、灵敏、高效等优点,在苹果品种鉴定中得到成功应用。
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