The major structural protein gene was cloned from Bombyx mori densovirus,Zhenjiang strain （BmDNV-ZJ）,sequenced and expressed in Escherichia *** gene consists of 1500 nucleotides and is predicted to code a protein of...
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The major structural protein gene was cloned from Bombyx mori densovirus,Zhenjiang strain （BmDNV-ZJ）,sequenced and expressed in Escherichia *** gene consists of 1500 nucleotides and is predicted to code a protein of 499 amino *** identity of 98.1% in nucleotide sequence and 98.6% of the predicted amino acid sequence are shared with its homologue,Bombyx mori densovirus （Yamanashi isolate） VD1-ORF2,*** gene was inserted into expression plasmid vector pET-28a and expressed in Escherichia coli BL21（DE3） strain under the inducible promoter *** expressed 53kD product could be detected by Western blotting with viral *** suggests that the gene we cloned is a structural protein gene.
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