A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-MS/MS) method for the simultaneous determination of aspirin (ASA) and its metabolite salicylic acid (SA) in human plasma has been...
详细信息
A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-MS/MS) method for the simultaneous determination of aspirin (ASA) and its metabolite salicylic acid (SA) in human plasma has been developed and *** plasma ASA and SA were extracted using a liquid-liquid extraction (LLE) and the sample extract was injected onto the LC-MS/MS *** limits of quantitation(LLOQ) for ASA and SA are both 25 ng/*** method allowed the reproducible and accurate quantification with the concentration rang of 25-10 000 ng/*** method could be applied to the quantitation aspirin and salicylic acid in human plasma.
Here we report a sensitive LC-MS/MS method capable of quantifying n-Butylphthalide (NBP) down to 0.5 ng/mL in rat and human hepatic microsome incubating *** method was validated over linear range of 0.5-4 000 ng/mL us...
详细信息
Here we report a sensitive LC-MS/MS method capable of quantifying n-Butylphthalide (NBP) down to 0.5 ng/mL in rat and human hepatic microsome incubating *** method was validated over linear range of 0.5-4 000 ng/mL using diazepam as the internal *** was simply prepared by protein precipitation method and analyzed on the LC-MS/MS,which was an API-4000 system equipped with ESI interface and a Symmetry C18 *** between-day and within-day precision and accuracy of quality control samples were ≤9.3% RSD% and ≤3.3% RE%.Sample stability (Stock solution,freeze/thaw,bench-top,short-term and long-term stability) were also *** method has been applied to drug-drug interaction study of NBP in rat and human hepatic microsome incubating solutions.
暂无评论