Objective To observe the efficiency and time course of gene expression and the safety of adenoviral vector mediated gene transfer in *** After soaking soluble stents in a high concentration of glucose solution conta...
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Objective To observe the efficiency and time course of gene expression and the safety of adenoviral vector mediated gene transfer in *** After soaking soluble stents in a high concentration of glucose solution containing Adv5-CMV (cytomegalovirus) (control group) or Adv5-CMV/LacZ (treatment group) for 30 minutes, the stents were inserted into the lumina of cut rat carotid arteries and end-to-end anastomoses of the cut carotid were performed with standard microvascular surgical techniques. On days 2, 7, 14, 28, 60 and 90 after gene transfer, anastomotic arteries of the two groups were observed. On days 7 and 14, the ascending aortas, hearts, brains, livers, lungs, spleens and kidneys of the treatment group were observed. All samples were analyzed for the presence of β-galactosidase activity and histochemical *** β-galactosidase activity was not detected in the carotid arteries of the control group and organs not directly exposed to adenoviral vector of the treatment group. The amount of β-galactosidase activity (×10-3?U/g tissue) in the treatment group on the 2nd, 7th, 14th, 28th, 60th and 90th day after gene transfer was 3.87, 11.38, 9.8, 6.43, 3.18 and 2.43, respectively. Microscopic examination of sections from vessels of the control group and from the aortas, hearts, brains, livers, lungs, spleens or kidneys of the treatment group revealed no X-gal staining. Microscopic examination of carotid arteries of the treatment group revealed blue-staining in all anastomotic arteries and in all layers of the arterial wall observed on days 7 and 14 after gene *** Adenoviral vector can effectively infect blood vessels in vivo. After adenoviral vector mediated direct gene transfer into anastomotic rat carotid arteries, recombinant gene expression began on day 2, peaked between days 7 and 14, prominently declined after day 28, and persisted at low levels more than three months. A recombinant gene could be delivered to a specific site by di
目的应用超声心动图比较肥厚型梗阻性心肌病患者经皮经腔间隔心肌消融术与室间隔心肌切除术的疗效。方法分别于经皮经腔间隔心肌消融术及室间隔心肌切除术前、后测量肥厚型梗阻性心肌病患者左室流出道压差,比较术前及术后压差。结果肥厚型梗阻性心肌病患者在进行经皮经腔间隔心肌消融术与室间隔心肌切除术后,左室流出道压差均较术前明显减低。经皮经腔间隔心肌消融术肥厚型心肌病患者术前左室流出道压差(99±19)mm Hg(1 mm Hg=0.133 kPa),术后降至(36±20) mm Hg(P<0.05)。进行室间隔心肌切除术肥厚型心肌病患者术前左室流出道压差(117±32) mm Hg,术后降至(28±17) mm Hg(P<0.05)。经皮经腔间隔心肌消融术与室间隔心肌切除术患者术后左室流出道压差差异无统计学意义[(36±20)mm Hg比(28±17)mm Hg]。结论经皮经腔间隔心肌消融术可以明显减低肥厚型梗阻性心肌病患者左室流出道压差,且与室间隔心肌切除术疗效相似。
目的探讨主动脉瘤手术患者术中脑氧饱和度的变化与脑部并发症发生的相关性,评价两种脑保护方法的效果。方法 30例主动脉瘤手术患者中主动脉夹层 Stanford A 型24例,StanfordB 型4例,升主动脉及主动脉弓部瘤1例,假性胸腹主动脉瘤1例。在...
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目的探讨主动脉瘤手术患者术中脑氧饱和度的变化与脑部并发症发生的相关性,评价两种脑保护方法的效果。方法 30例主动脉瘤手术患者中主动脉夹层 Stanford A 型24例,StanfordB 型4例,升主动脉及主动脉弓部瘤1例,假性胸腹主动脉瘤1例。在深低温停循环(DHCA)合并选择性顺行脑灌注(ASCP)下行主动脉弓部替换22例(ASCP组);在单纯 DHCA 下行胸降主动脉替换8例(DHCA 组)。于术中应用近红外光谱分析技术(NIRS)进行持续脑氧饱和度(TOI)监测。结果出现脑部并发症患者8例。并发症组与非并发症组术中转机前 TOI 分别为70%±5%和69%±8%,分别降至58%±8%和55%±8%,两组间差异无统计学意义(P>0.05)。停循环期间 DHCA 组 TOI 从77%±6%降至52%±7%,ASCP 组 TOI 从75%±6%降至71%±7%;两组间 TOI 的下降程度差异有统计学意义(P<0.05)。结论轻度的 TOI 降低与脑部并发症的发生无明显相关性。DHCA 合并ASCP 与单纯的 DHCA 相比,具有更好的脑保护效果。
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