目的:建立一种用SYBR Green Ⅰ荧光染料检测乙肝病毒的实时聚合酶链反应(PCR)方法。 方法:应用SYBR Green Ⅰ染料掺入双链DNA的原理，并对实时荧光定量PCR(real-time PCR)反应条件进行优化，验证方法的特异性、敏感性和稳定性，定量...
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目的:建立一种用SYBR Green Ⅰ荧光染料检测乙肝病毒的实时聚合酶链反应(PCR)方法。
方法:应用SYBR Green Ⅰ染料掺入双链DNA的原理，并对实时荧光定量PCR(real-time PCR)反应条件进行优化，验证方法的特异性、敏感性和稳定性，定量检测HBV DNA水平。以抗乙肝药物拉米夫定为例证明该方法。
结果:建立了定量检测HBV DNA的方法。
结论:应用SYBR Green Ⅰ对HBV DNA的相对定量具有简便、快速、重复性好、特异性强的特点，适用于抗HBV药物的大样品筛选及评价优化工作，并可发展成为高通量技术。
A reverse-phase HPLC method was developed for the simultaneous separation and determination of five bioactive phenolic acids,yunnaneic acid E,rosmarinic acid,lithospermic acid,salvianolic acid B and salvianolic acid A...
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A reverse-phase HPLC method was developed for the simultaneous separation and determination of five bioactive phenolic acids,yunnaneic acid E,rosmarinic acid,lithospermic acid,salvianolic acid B and salvianolic acid A in eight different samples of Salvia yunnanensis collected in Yunnan *** comparison,the sample of Salvia miltiorrhiza was included. All the samples were extracted for 60 min with 50%methanol in an ultrasonic *** optimal separation was achieved on a YMC-Pack Pro C18 column,with a gradient of 0.1%（v/v） phosphoric acid and acetonitrile,at a flow rate of 1.0 mL/min and at a detection wavelength of 280 *** separation was obtained within 65 min for five bioactive phenolic *** calibration curves showed good linearity（r^2〉0.999） within test *** relative standard deviation of the method was less than 5%for intra- and inter-day *** mean recovery of the method was in the range from 97%to 104%,with RSD less than 5%.This assay was successfully applied to the quantitative determination of five bioactive phenolic acids in nine resource samples. The results showed that the developed HPLC assay was suitable for the quality control of *** and it can be used to differentiate *** from ***.
2009年被美国“科学”杂志评为全球十大科学发现之一，是特异作用TOR（Target Of Rapamycin，TOR）的免疫抑制剂雷帕霉素（rapamycin）能明显地延长小鼠的寿命。这项激动人心的研究结果不仅使老年医学研究者进一步坚定了药物抗衰老的信...
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2009年被美国“科学”杂志评为全球十大科学发现之一，是特异作用TOR（Target Of Rapamycin，TOR）的免疫抑制剂雷帕霉素（rapamycin）能明显地延长小鼠的寿命。这项激动人心的研究结果不仅使老年医学研究者进一步坚定了药物抗衰老的信心，同时也促使人们重新审视TOR信号通路与衰老的关系，
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