RNA interference(RNAi) is emerging as one of promising strategies for tumor therapy,especially for inhibiting tumor ***,an efficient and safety non-virus delivery system for clinical siRNA therapy is still a major ***...
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RNA interference(RNAi) is emerging as one of promising strategies for tumor therapy,especially for inhibiting tumor ***,an efficient and safety non-virus delivery system for clinical siRNA therapy is still a major *** applications of cationic non-viral vectors are limited because of their low transfection efficiency,especially in the serum containing mediums and in vivo *** difficulty of lysosome escape and nonspecific adsorption of serum proteins are considered the main *** this talk,I'd like to introduce several recent approaches developed by our group to overcome these barriers for siRNA ***,in order to improve the escape efficiency of siRNA from lysosomes and endosomes,we prepared a novel photosensitive carrier(Ceo-Dex-NEb) to delivery siRNA into *** result shows that visible light can trigger ROS generation,promote the escaping of carrier-siRNA complex from lysosome,and increase the interference efficiency in ***,to prolong the function period of siRNA and protect it from serum conditions,we designed a kind of cationized gelatin nanoparticles as an efficient siRNA carrier,and evaluated the silencing efficacy to CXCR4,which is highly expressed in various tumor cells and involved in the tumor ***,the role and mechanisms of the serum in the culture medium will be *** serum is one crucial factor that compromises RNAi efficiency of cationized non-viral gene ***,the roles of serum in siRNA delivery process remain *** the present work,we took one cationic polymer,pullulan chemically modified by spermine(termed as pullulan-spermine,Ps),as a siRNA carrier model to investigate the effects of serum on key steps in siRNA delivery including formation of Ps and siRNA polyplexes(Ps-siRNA),cellular uptake,lysosomal escape,and *** demonstrated that modulating serum concentration can regulate the size and morphology of the siRNA-Ps complex,thus a
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