Objective To investigate the effect of aminoglycosides on the expression of nonsense *** HERG channel and mutant currents were recorded by whole-cell patch clamp *** rescue was applied by culturing the cells in 400μg...
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Objective To investigate the effect of aminoglycosides on the expression of nonsense *** HERG channel and mutant currents were recorded by whole-cell patch clamp *** rescue was applied by culturing the cells in 400μg/mL G-418 or gentamicin for 24 *** Current densities were significantly reduced in cells expressing R1014X and W927X mutants compared to those of cells expressing wild type(WT) HERG.R863X and E698X mutants failed to generate any typical HERG *** mean peak tail current density of R1014X mutant was significantly lower than that of WT[(3.9±1.4)
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