Objective To explore circulating biomarkers for screening the invasiveness of non-functioning pituitary adenomas(NF-PAs).Methods The exosomal RNAs were extracted from serum of patients with invasive NF-PA(INF-PA)or no...
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Objective To explore circulating biomarkers for screening the invasiveness of non-functioning pituitary adenomas(NF-PAs).Methods The exosomal RNAs were extracted from serum of patients with invasive NF-PA(INF-PA)or noninvasive NF-PA(NNF-PA).Droplet digital PCR was adapted to detect the mRNA expression of candidate genes related to tumor progression or invasion,such as cyclin dependent kinase 6(CDK6),ras homolog family member U(RHOU),and spire type actin nucleation factor 2(SPIRE2).Student’s t-test was used to analyze the statistical difference in the mRNA expression of candidate genes between the two *** operating characteristic(ROC)curve was used to establish a model for predicting the invasiveness of *** accuracy,sensitivity,specificity and precision of the model were then obtained to evaluate the diagnostic *** CDK6(0.2600±0.0912 vs.0.1789±0.0628,t=3.431,P=0.0013)and RHOU mRNA expressions(0.2696±0.1118 vs.0.1788±0.0857,t=2.946,P=0.0052)were upregulated in INF-PAs patients’serum exosomes as compared to *** CDK6,the area under the ROC curve(AUC)was 0.772(95%CI:0.600-0.943,P=0.005),the accuracy,sensitivity,specificity and precision were 77.27%,83.33%,75.00%and 55.56%to predict the invasiveness of *** RHOU,the AUC was 0.757(95%CI:0.599-0.915,P=0.007),the accuracy,sensitivity,specificity and precision were 72.73%,83.33%,68.75%and 50.00%.In addition,the mRNA levels of CDK6 and RHOU in serum exosomes were significantly positively correlated(r=0.935,P<0.001).After combination of the cut-off scores of the two genes,the accuracy,sensitivity,specificity and precision were 81.82%,83.33%,81.25%and 62.50%.Conclusions CDK6 and RHOU mRNA in serum exosomes can be used as markers for predicting invasiveness of *** of the two genes performs better in distinguishing INF-PAs from *** results indicate CDK6 and RHOU play important roles in the invasiveness of NF-PAs,and the established diagnostic method is valua
前期相关工作表明,MRG(Myostatin Related Gene)作为TGF-β超家族Myostatin的下游靶基因,在Myostatin处理的鸡原代成肌细胞中表达上调;Myostatin是成肌细胞增殖和分化的负调控因子,而MRG的功能目前知之甚少,在细胞中过表达MRG可以抑制...
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前期相关工作表明,MRG(Myostatin Related Gene)作为TGF-β超家族Myostatin的下游靶基因,在Myostatin处理的鸡原代成肌细胞中表达上调;Myostatin是成肌细胞增殖和分化的负调控因子,而MRG的功能目前知之甚少,在细胞中过表达MRG可以抑制肌细胞和几种其它非肌细胞包括癌细胞的增殖过
目的研究切断面神经后热休克蛋白27(heat shock protein 27,HSP27)在面运动核团内表达的变化情况;探讨面神经损伤后面神经功能恢复的分子机制。方法用随机数字表法将30只Wistar大鼠分为3组:实验组、手术对照组、空白对照组,每组10只;在...
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目的研究切断面神经后热休克蛋白27(heat shock protein 27,HSP27)在面运动核团内表达的变化情况;探讨面神经损伤后面神经功能恢复的分子机制。方法用随机数字表法将30只Wistar大鼠分为3组:实验组、手术对照组、空白对照组,每组10只;在切断右侧面神经术前和术后的第1、2、3、4天,获取不同组内2只大鼠右侧的面运动核团,从核团组织中提取包含热休克蛋白27在内的上清液,用Western blotting方法测定上述不同实验组的组织上清液内热休克蛋白27的表达。结果切断面神经术后第1天实验组,大鼠面运动核团内热休克蛋白27表达比切断前有明显提高,术后第2天表达达到高峰,术后第3天表达开始下降,术后第4天表达较术后第3天继续下降,但仍高于术前;手术对照组和空白对照组HSP27的表达无明显变化。结论切断面神经后,面运动核团内热休克蛋白27表达上调,术后第2天表达达到高峰,之后递减到术后第4天,但表达始终高于术前。
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