以115个欧洲黑杨(Populus nigra L.)无性系为材料,利用TaqMan技术分析了欧洲黑杨基因资源参与木质素和纤维素合成的酶(4CL、PAL和CesA2)的单核苷酸多态性,并对分型的SNPs与木材材性性状(物理性状:基本密度、纤维长、纤维宽、微纤丝角;...
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以115个欧洲黑杨(Populus nigra L.)无性系为材料,利用TaqMan技术分析了欧洲黑杨基因资源参与木质素和纤维素合成的酶(4CL、PAL和CesA2)的单核苷酸多态性,并对分型的SNPs与木材材性性状(物理性状:基本密度、纤维长、纤维宽、微纤丝角;化学性状:木质素含量、纤维素含量、α纤维素含量等)进行了相关分析。结果如下:(1)在对4CL、PAL和CesA2等3个基因进行检测时,共获得27个SNPs标记,对其中转换(A-G,C-T)有17个位点,颠换(A-C,G-C,G-T,A-T等)有10个位点;(2)对其中的3个SNPs进行了分型,分别记作SNP1、SNP2和SNP3;(2)对已经分型SNPs位点与材性性状进行方差分析,结果显示,3个SNPs中只有SNP1与4年生欧洲黑杨综纤维素含量显著相关,表现为负效应,贡献率为11.11%;(3)对欧洲黑杨4CL基因的SNP1标记的不同基因型所对应的材性性状进行方差分析,结果显示基因型为CC和CT的欧洲黑杨相对于基因型为TT的欧洲黑杨有较高的纤维素含量。
A mating design,including full-sib and half-sib Catalpa bungei was *** germination ability was *** temperature,soaking and lighting time and pretreatment that could influence on Catalpa bungei seed germination were **...
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A mating design,including full-sib and half-sib Catalpa bungei was *** germination ability was *** temperature,soaking and lighting time and pretreatment that could influence on Catalpa bungei seed germination were *** result showed that the difference of germination rate and energy among different mating combinations was extremely *** influence of the individual plant to the germination rate was lower than that of different *** germination rate of the combination *** and *** was always *** germination rate and energy of two set of reciprocal crosses were very *** germination rate was the highest for soaking the seed for 24 hours at a constant temperature of 25 ℃ then germinating it at a changing temperature 20-30 ℃,the difference was *** result,an 8-hour-long lighting daily was more advantaged for the germination of the seed than that in a completely dark condition;the germinating rate of the seed stored at a temperature of 5 ℃ for a week after soaking is also much higher than that stored at a low temperature.
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