采用热水提取、用Savage法除蛋白,过氧化氢法脱色得到杜仲叶粗多糖(crude polysaccharide from leaves of eucommia ulmoides oliver)。经Sepharose CL-6B分离纯化得到杜仲多糖的一个组分EOP-1,通过HPSEC分析其纯度及测定分子量,表明该...
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采用热水提取、用Savage法除蛋白,过氧化氢法脱色得到杜仲叶粗多糖(crude polysaccharide from leaves of eucommia ulmoides oliver)。经Sepharose CL-6B分离纯化得到杜仲多糖的一个组分EOP-1,通过HPSEC分析其纯度及测定分子量,表明该多糖约为6.0×105 D。部分酸水解和甲基化分析,结合GC-MS测试手段,对杜仲多糖EOP-1的糖链结构进行了研究。结果显示,EOP-1的糖残基主要由D-GalpA、D-Glcp、D-Galp、L-Araf和L-Rhap组成,主链由1,4-D-GalpA连接,为半乳糖醛酸聚糖,侧链主要由1,4-D-Galp、1,6-D-Galp、1,5-L-Araf、1,2-L-Rhap构成,其相对摩尔百分比为45.11∶35.9∶0.9∶10.5∶6.9。
The research adopts open sampling method and TCT-GC/MS(Thermodesorption Cold Trap-Gas Chromatography/Mass Spectrometery) apparatus to collect and analyze the volatile organic compounds(VOCs) of Platycladus orientalis ...
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The research adopts open sampling method and TCT-GC/MS(Thermodesorption Cold Trap-Gas Chromatography/Mass Spectrometery) apparatus to collect and analyze the volatile organic compounds(VOCs) of Platycladus orientalis forest,with the intention of researching the compounds and diurnal variation of VOCs of *** forest in natural state in Beijing Western Hills in *** results are as follows:(1) There are 160 kinds(11 categories) of volatile organic compounds,containing Alkyl Hydrocarbon,Ketone,Alcohol,Acid,Aldehyde,Alkene,Ester,Aromatic hydrocarbon,Ether,Hydroxybenzene and *** content of Alkyl Hydrocarbon,Ketone,Acid and Aldehyde occupy a greater percentage among all compounds.(2) The diurnal variation curve of total volatile organic compounds(TVOC) is characterized as "one peak at 11:00(36.44%) and one vale at 3:00(12.76%)".Generally,the concentration at daytime is higher than that of night,and higher in morning than in afternoon,with the maximum at noon.(3) The change of Alkyl Hydrocarbon concentration is greater in the morning and about midday,less in the afternoon and *** diurnal variation curve of Ketone concentration appears as "three peaks and three vales".1:00,5:00—7:00 and 11:00 are peak time,3:00,9:00 and 17:00 are vale *** to Acid,its concentration appears two obvious phases: the higher phase is from 9:00 to 17:00,with the maximum at 11:00,and the lower phase is from 19:00 to 7:00,with the minimum at 5:*** Aldehyde concentration,17:00 and 19:00 are the higher phases and also has obvious difference with other time,while the lower phrase has no obvious difference with each other.(4) Alkene is the most important compound to human *** concentration appears as "one peak" at 15:00,which has obvious difference with other ***,its content at daytime is higher than that at night,and higher in afternoon than in morning.
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