[Objective] The aim was to research the relationship and genetic diversity of Indocalamus. [Method] Using 13 samples of Indocalamus and 3 samples of Sasa as materials, the intergenic regions of trnL-trnF gene in chlor...
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[Objective] The aim was to research the relationship and genetic diversity of Indocalamus. [Method] Using 13 samples of Indocalamus and 3 samples of Sasa as materials, the intergenic regions of trnL-trnF gene in chloroplast were amplified by PCR, and sequence analysis and phylogenetic trees construction were carried out. [Result] Using the universal primer, the intergenic regions of trnL-trnF were amplified, the lengths of the segments varied from 1 008 bp to 1 103 bp, of which 940 bp was compared. The dendrogram of trnL-trnF sequences showed that Indocalamus and Sasa were clustered together and they were homologous by 99%. All the samples were divided into five groups, the first group included 12 samples such as Indosalamus pedalis, I. pumilus, I. victorialis, I. longiauritus, I. tessellatus, Sasa sinica, Sasa pygmaea, I. barbatus, I. guangdongensis, I. herklotsii, I. Hirtivaginatus and S. fortunei. I. decorus, I. lacunosus, I. Latifolius and *** were respectively divided into four groups. [Conclusion] The high homology of all samples showed the low evolution speed and little information sites which suggested that the phylogeny of Indocalamus could not be well resolved by the intergenic region of trnL-trnF.
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