In this paper,a protocol for efficient shoot regeneration was successfully developed from hypocotyl explants of Euonymus fortunei *** factors that influenced shoot regeneration such as different combinations of plant ...
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In this paper,a protocol for efficient shoot regeneration was successfully developed from hypocotyl explants of Euonymus fortunei *** factors that influenced shoot regeneration such as different combinations of plant growth regulators,types of medium and inoculation ways were studied in order to establish an efficient plant regeneration for *** results showed that hypocotyl explants were horizontally cultured on a basic medium composed of MS medium supplemented with 0.5 mg·L-1 BAP and 0.01 mg·L-1 NAA for induction and development of adventitious ***-four percent of regeneration frequency and 5.1 shoots per explants were obtained after 30 days of *** shoots proliferated efficiently on a shoot multiplication medium consisting of MS medium containing 1.0 mg·L-1 BAP and 0.1 mg·L-1 *** were rooted on a rooting medium made up of MS medium enriched with 0.5 mg·L-1 IBA and 0.5 mg·L-1 *** hardening,90% of plants were successfully established under greenhouse *** observation revealed that shoot primordium originated from subepidermal cells of hypocotyl explants and directly developed into adventitious shoots without callus formation.
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