At present,the progress of the study and application of forest plant disease was impeded by the lack of the internet resources of research achievements and control methods of forest plants *** a forest plant disease i...
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At present,the progress of the study and application of forest plant disease was impeded by the lack of the internet resources of research achievements and control methods of forest plants *** a forest plant disease information manage system based on internet were *** forest plant disease information website was established on the basis of HTML,ASP,Javascript and Microsoft Access *** sources of the database were Chinese forest plant disease occurred severely in recent decades and the forest plant disease specimen of Chinese Academy of *** website was divided into six basic function modules,including the home page,the research group information,the guestbook,the forest plant disease information management system,the forest plant disease specimen management system and the forest plant disease diagnose *** forest plant disease information could be easily queried and browsed through supported fields with these function ***,the discussions about forest plant disease could be done with the guestbook or *** information and achievements of the research group were also showed through the website.
杨树受损伤后能诱导一些基因的表达,其编码的蛋白质可能在杨树的防卫反应中起一定作用。用PCR方法从新疆杨叶片中克隆出一个损伤诱导型Kun itz胰蛋白酶抑制剂基因PaTI1。序列分析表明:此基因不含内含子,其翻译起点上游具有‘TATA’和‘CCAAT’等转录控制元件,包含的阅读框架能编码一个长为213个氨基酸的多肽。此多肽与克隆自美洲山杨的PtTI2和PtTI1氨基酸序列同源性最高,分别为95%和80%,其N端存在一长度为27个氨基酸的信号肽。将此基因以融合蛋白的形式在大肠杆菌中进行表达,纯化后的融合蛋白对胰蛋白酶的活性有抑制作用,每8.5μg融合蛋白可完全抑制1μg牛胰蛋白酶的活性。W estern b lot分析表明融合蛋白与PtTI2特异的抗体之间有明显的血清学反应。
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