Soil microbial features on Chai-He Zinc-lead Mine wasteland and non-mine soils were *** results showed that soil micoflora changed obviously and total of major soil microbes *** amount of microbe decreased by 83.03 %...
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Soil microbial features on Chai-He Zinc-lead Mine wasteland and non-mine soils were *** results showed that soil micoflora changed obviously and total of major soil microbes *** amount of microbe decreased by 83.03 %～93.65 % in top soil（0～20 cm） compared with that of the non-mine *** soils possessed obviously different microbial features higher in microbial basal respiration strength,and microbial ecophysiological Cmic/Corg and qCO2,but lower in micobial biomass compared with the *** of these might lead to the impairment of turnover and cycle velocity of C,N nutrition element in ***’s one major problem which should be solved in the procession of reclaimed mine-soil.
杨树受损伤后能诱导一些基因的表达,其编码的蛋白质可能在杨树的防卫反应中起一定作用。用PCR方法从新疆杨叶片中克隆出一个损伤诱导型Kun itz胰蛋白酶抑制剂基因PaTI1。序列分析表明:此基因不含内含子,其翻译起点上游具有‘TATA’和‘CCAAT’等转录控制元件,包含的阅读框架能编码一个长为213个氨基酸的多肽。此多肽与克隆自美洲山杨的PtTI2和PtTI1氨基酸序列同源性最高,分别为95%和80%,其N端存在一长度为27个氨基酸的信号肽。将此基因以融合蛋白的形式在大肠杆菌中进行表达,纯化后的融合蛋白对胰蛋白酶的活性有抑制作用,每8.5μg融合蛋白可完全抑制1μg牛胰蛋白酶的活性。W estern b lot分析表明融合蛋白与PtTI2特异的抗体之间有明显的血清学反应。
44 isolates of fungi were isolated from the xylem of the Pinus thunbergii samples(healthy stem,weak stem and withered stem)infected by the pine wilt disease caused by pine wood nematode,Bursaphelenchus xylophilus,from...
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44 isolates of fungi were isolated from the xylem of the Pinus thunbergii samples(healthy stem,weak stem and withered stem)infected by the pine wilt disease caused by pine wood nematode,Bursaphelenchus xylophilus,from infected trees at Zhoushan and Pinghu of Zhejiang Province in China,and these isolates were identified as:Trichoderma spp.,Penicillium spp.,Pestalotia *** identified isolates took 70.62% of total isolates of the *** the 12 isolates unidentified,one sporiferous fungus was found,the other 11 isolates were *** *** the dominant fungus in ***,the total rate was 53.34%,followed by Penicillium spp.,18.89%.
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