Crotalaria witches’-broom phytoplasma (CrWB) collected from Hainan Province,was classified and identified by sequencing techniques and Virtual *** different type universal primer pairs of phytoplasma were used to amp...
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Crotalaria witches’-broom phytoplasma (CrWB) collected from Hainan Province,was classified and identified by sequencing techniques and Virtual *** different type universal primer pairs of phytoplasma were used to amplify a 1.8 kb fragment of 16S rDNA and a 1.3 kb fragment of rp *** PCR-amplified products were cloned and *** results of sequencing and homologuous comparison with other phytoplasmas showed that Crotalaria witches’-broom phytoplasma shared 99.9 % similarity with peanut witches’-broom phytoplasma in 16S rRNA gene and 99.9% in rp *** witches’-broom phytoplasma is sorted into Peanut witches’-broom group(16SrII),16SrII-A,according to the results of Virtual RFLP of 16S rDNA,phylogenetic analysis of 16S rRNA and rp gene,which is related to *** australasiae.
The paper had analyzed landscape pattern of habitat of Sichuan golden monkey in Baihe Nature *** result showed:① there were altogether 5 069.00 patches at habitat of Sichuan golden monkey in Baihe Nature Reserve,the ...
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The paper had analyzed landscape pattern of habitat of Sichuan golden monkey in Baihe Nature *** result showed:① there were altogether 5 069.00 patches at habitat of Sichuan golden monkey in Baihe Nature Reserve,the patch density was 2 595 p/hm2,average patch area was 3.20 hm2,Shannon's diversity index was 2.49,patch richness was 68.00,total length of edge was 19 011 119.91 m,edge density was 97.34 m/km2,and landscape shape index was 35.19.② The quality of habitat of Sichuan golden monkey reduced,patches at the habitat were seriously fragmentized,and it urgently needed to enhance protection and increase *** paper suggested connecting fragmented habitats through establishing habitat corridor in nature reserve;strengthening resources protection and strictly examining development projects of tourist products;increasing scientific research and capital investment;adopting effective measures to ameliorate local community environment and relieving the pressure of nature reserve caused by people;intensifying relevant publicity and education,so as to improve quality of habitat of Sichuan golden monkey in the nature reserve and effectively protect this valuable species.
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