In this paper,a protocol for efficient shoot regeneration was successfully developed from hypocotyl explants of Euonymus fortunei *** factors that influenced shoot regeneration such as different combinations of plant ...
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In this paper,a protocol for efficient shoot regeneration was successfully developed from hypocotyl explants of Euonymus fortunei *** factors that influenced shoot regeneration such as different combinations of plant growth regulators,types of medium and inoculation ways were studied in order to establish an efficient plant regeneration for *** results showed that hypocotyl explants were horizontally cultured on a basic medium composed of MS medium supplemented with 0.5 mg·L-1 BAP and 0.01 mg·L-1 NAA for induction and development of adventitious ***-four percent of regeneration frequency and 5.1 shoots per explants were obtained after 30 days of *** shoots proliferated efficiently on a shoot multiplication medium consisting of MS medium containing 1.0 mg·L-1 BAP and 0.1 mg·L-1 *** were rooted on a rooting medium made up of MS medium enriched with 0.5 mg·L-1 IBA and 0.5 mg·L-1 *** hardening,90% of plants were successfully established under greenhouse *** observation revealed that shoot primordium originated from subepidermal cells of hypocotyl explants and directly developed into adventitious shoots without callus formation.
The main nutrient content and mineral element content in fresh bamboo shoots of Pleioblastus amarus(Keng)Keng f.,*** *** et *** ***(McClure) *** et *** were *** results show that the water content in fresh bamboo shoo...
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The main nutrient content and mineral element content in fresh bamboo shoots of Pleioblastus amarus(Keng)Keng f.,*** *** et *** ***(McClure) *** et *** were *** results show that the water content in fresh bamboo shoots of ***,*** and *** is 91.32%,90.87% and 91.69%,respectively,and the difference is not *** crude fiber content is 13.9,18.8 and 19.3 g·kg-1,crude fat content is 8.90,7.20 and 6.60 g·kg-1,ash content is 10.90,4.10 and 7.60 g·kg-1,VC content is 0.133,0.154 and 0.087 g·kg-1,crude protein content is 21.2,19.5 and 14.0 g·kg-1,reducing sugar content is 0.80,1.20 and 2.20 g·kg-1,respectively,and the significant difference exists among the three species.K content in fresh bamboo shoots of the three species is the highest(1 152.8-1 628.1 μg·g-1),and Ca and Mg contents are 63-86 μg·*** content in that of ***,*** and *** is 22.4,21.9 and 20.3 μg·g-1,respectively,and the difference is not ***,there is 16.2 μg·g-1 Fe and 0.30 μg·g-1 Cr in fresh bamboo shoots of ***,but Fe and Cr can not be detected in that of *** and ***.
The light harvesting chlorophyll a/b-binding protein is one of key proteins in the transformation from light energy to chemical energy. An open reading frame coding precursor protein of cab gene was cloned from the fi...
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The light harvesting chlorophyll a/b-binding protein is one of key proteins in the transformation from light energy to chemical energy. An open reading frame coding precursor protein of cab gene was cloned from the first strand of bamboo cDNA through RT-PCR methods,and named as cab-PhE1 (cab gene 1 from Phyllostachys edulis EF207229). The sequence analysis showed that the deduced polypeptide was highly homologous to some other CAB proteins from monocotyledon,and the gene belonged to lhcb2 family. Tissue specific expression showed that cab-PhE1 expressed higher in leaf than sheath and stem. The prokaryotic expression vector of cab-PhE1 gene encoding the mature protein was constructed by subcloning the fragment into pET-23 a and was expressed in Escherichia coli induced by IPTG. The molecular weight of the induced protein was about 28 ku,approximate to that of the mature protein. This work is a key to the further research on in vitro reconstitution of light-harvesting Chl a/b complexes.
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