In this study,we presented an enrichment-free approach for the sensitive analysis of protein phosphorylation in minute amounts of samples,such as purified protein *** method takes advantage of the high sensitivity of ...
In this study,we presented an enrichment-free approach for the sensitive analysis of protein phosphorylation in minute amounts of samples,such as purified protein *** method takes advantage of the high sensitivity of parallel reaction monitoring (PRM).Specifically,low confident phosphopeptides identified from the data dependent acquisition (DDA) dataset were used to build a pseudo-targeted list for PRM analysis to allow the identification of additional phosphopeptides with high confidence.
微柱液相色谱(micro-LC)是指采用内径为0.l mm～1.00 mm 色谱柱的液相色谱,流量一般在1～100μL/min,相对于常规液相色谱具有流动相消耗少、进样量小等优点.与电喷雾离子源(ESI)的质谱联用时,由于流量较小,产生的喷雾液滴较小,基质效应...
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微柱液相色谱(micro-LC)是指采用内径为0.l mm～1.00 mm 色谱柱的液相色谱,流量一般在1～100μL/min,相对于常规液相色谱具有流动相消耗少、进样量小等优点.与电喷雾离子源(ESI)的质谱联用时,由于流量较小,产生的喷雾液滴较小,基质效应也相应较低,能够提高待分析物的离子化效率,从而提高检测灵敏度.
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