Oxygen/glucose deprivation （OGD） has been widely used as an in vitro model of focal ischemia, where the blood flow is severely reduced and neurons rapidly die. However, adjacent to the focal region is ‘penumbra＇, ...
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Oxygen/glucose deprivation （OGD） has been widely used as an in vitro model of focal ischemia, where the blood flow is severely reduced and neurons rapidly die. However, adjacent to the focal region is ‘penumbra＇, where residual blood flow remains oxygen and glucose supplies are at low levels. To model this pathological genesis, we developed a partial OGD （pOGD） protocol in a rat brain slice. This model met two requirements： oxygen was partially deprived and glucose was reduced in the perfusion buffer. Therefore we investigated the effect of pOGD on gama-aminobutyric acid （GABAA） receptor-mediated inhibitory postsynaptic currents （IPSCs） in CA1 neurons of a hippocampal slice through whole-cell patch-clamp technique. We found that the amplitude and decay time of IPSCs were increased immediately during pOGD treatment. And the enhancement of IPSCs amplitude resulted from an increase of the synaptic conductance without a significant change in the reversal potential of chloride. These results suggested that the nervous system could increase inhibitory neurotransmission to offset excitation by homeostasis mechanisms during the partial oxygen and glucose attack.
应激是生物体对环境改变或压力的反应。当今神经科学的这个研究领域中,技术上通常用皮质激素(glucocorticoids)在血液中的水平高低评价行为应激(Kim and Diamond,2002)。众所周知,大脑并不是对任何事件产生同样牢固的记忆。从进化的角...
详细信息
应激是生物体对环境改变或压力的反应。当今神经科学的这个研究领域中,技术上通常用皮质激素(glucocorticoids)在血液中的水平高低评价行为应激(Kim and Diamond,2002)。众所周知,大脑并不是对任何事件产生同样牢固的记忆。从进化的角度来看,大脑对重要的事件如应激等产生牢固的记忆而不是对所有的事件产生牢固记忆更具有生物学意义。利用应激研究学习记忆的机理尤其是记忆的永久性储存将具有独特的科学价值,因为应激相关的牢固记忆在许多精神疾病中非常明显。这些精神疾病包括:创伤后应激综合征(PTSD)、抑郁症、强迫症以及毒品成
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