Thymosin α 1 was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α 1 was synthesized with the...
详细信息
Thymosin α 1 was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α 1 was synthesized with the solid-phase method, by using the base-liable Fmoc group to protect the α-amino acid and N,N′-diisopropylcarbodiimide (DIC) as the coupling regent. The crude product was analyzed by reversed-phase high performance liquid chromatography, SDS-PAGE and liquid chromatography/mass *** synthesis yield was 84.89%. The analytical results indicated that the crude product contained much thymosin α 1, and the purity was 50.3%. DIC solid-phase chemical synthesis of thymosin α 1 was applicable, and the yield was improved greatly compared to other known methods.
Fe2＋ oxidation by Acidithiobacillus ferrooxidans（***） under different solid contents by adding inert Al2O3 powder was examined in rotating-drum and stirred-tank *** results show that the bioactivity of *** in the s...
详细信息
Fe2＋ oxidation by Acidithiobacillus ferrooxidans（***） under different solid contents by adding inert Al2O3 powder was examined in rotating-drum and stirred-tank *** results show that the bioactivity of *** in the stirred-tank is higher than that in the rotating-drum in the absence of Al2O3 powder,but the biooxidation rate of Fe2＋ decreases markedly from 0.23 g/（L·h） to 0.025 g/（L·h） with increasing the content of Al2O3 powder from 0 to 50%（mass fraction） in the stirred-tank probably due to the deactivation of *** resulting from the collision and friction of solid *** increase in Al2O3 content has a little adverse effect on the bioactivity of *** in the rotating-drum due to different mixing mechanisms of the two *** biooxidation rate of Fe2＋ in the rotating-drum is higher than that in the stirred-tank at the same content of Al2O3 powder,especially at high solid *** higher bioactivity of *** can be maintained for allowing high solid content in the rotating-drum reactor,but its application potential still needs to be verified further by the sulfide bioleaching for the property differences of Al2O3 powder and sulfide minerals.
暂无评论