恩拉霉素作为多肽类抗生素,是一种新型、安全的饲料添加剂。本文建立了一条基于大孔树脂初纯和反相色谱精制的分离纯化工艺。该工艺路线首先使用AB-8大孔树脂在0.012 mol/L盐酸溶液-甲醇(50:50,V/V)缓冲液条件下洗脱实现恩拉霉素初步纯化,再使用制备型C18反相色谱柱在0.05 mol/L磷酸二氢钠-乙腈(70:30,V/V)(p H 4.5)缓冲液洗脱下实现恩拉霉素a和b的有效分离,a、b两个组分纯度分别达到98.5%和98.0%,a和b两种有效成分的总收率为29.2%。本研究为恩拉霉素a和b两种纯品的制备以及高纯度恩拉霉素产品的生产提供了参考。
Fe2＋ oxidation by Acidithiobacillus ferrooxidans（***） under different solid contents by adding inert Al2O3 powder was examined in rotating-drum and stirred-tank *** results show that the bioactivity of *** in the s...
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Fe2＋ oxidation by Acidithiobacillus ferrooxidans（***） under different solid contents by adding inert Al2O3 powder was examined in rotating-drum and stirred-tank *** results show that the bioactivity of *** in the stirred-tank is higher than that in the rotating-drum in the absence of Al2O3 powder,but the biooxidation rate of Fe2＋ decreases markedly from 0.23 g/（L·h） to 0.025 g/（L·h） with increasing the content of Al2O3 powder from 0 to 50%（mass fraction） in the stirred-tank probably due to the deactivation of *** resulting from the collision and friction of solid *** increase in Al2O3 content has a little adverse effect on the bioactivity of *** in the rotating-drum due to different mixing mechanisms of the two *** biooxidation rate of Fe2＋ in the rotating-drum is higher than that in the stirred-tank at the same content of Al2O3 powder,especially at high solid *** higher bioactivity of *** can be maintained for allowing high solid content in the rotating-drum reactor,but its application potential still needs to be verified further by the sulfide bioleaching for the property differences of Al2O3 powder and sulfide minerals.
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