目的:建立一种重组高密度脂蛋白(reconstituted high density lipoprotein,rHDL)载p53基因纳米粒的制备方法。方法:采用阳离子脂质材料包裹、压缩p53基因,形成复合物,考察其粒径、电位、体外稳定性;薄膜分散法制备rHDL载基因纳米粒,并研...
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目的:建立一种重组高密度脂蛋白(reconstituted high density lipoprotein,rHDL)载p53基因纳米粒的制备方法。方法:采用阳离子脂质材料包裹、压缩p53基因,形成复合物,考察其粒径、电位、体外稳定性;薄膜分散法制备rHDL载基因纳米粒,并研究rHDL载基因纳米粒的粒径、电位、形态、包封率和血清稳定性。结果:选用双十八烷基二甲基溴化铵(dimethyldioctadecylammonium bromide,DODAB)压缩p53基因形成DODAB/p53复合物;在最优N/P比时,该复合物粒径为(102.0±0.7)nm,电位为(10.50±0.75)mV,复合物仅在高浓度肝素条件下出现解聚,且其血清稳定性良好。rHDL能有效包载上述复合物形成rHDL载基因纳米粒,该纳米粒粒径为(104.3±5.8)nm,电位为(-10.70±3.24)mV,外观呈球形结构,p53基因包封率为78.09%,且血清稳定性良好。结论:成功制备了rHDL载p53基因纳米粒,且该纳米粒具有良好的理化性质及体外稳定性,为其后期体内外抗肿瘤作用的研究打下了实验基础。
A multifunctional copolymer-anticancer conjugate chitosan-graft-polyethyleneimine-candesartan(CPC)containing low molecular weight chitosan(CS)backbone and polyethyleneimine(PEI)arms with candesartan(CD)conjugated via ...
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A multifunctional copolymer-anticancer conjugate chitosan-graft-polyethyleneimine-candesartan(CPC)containing low molecular weight chitosan(CS)backbone and polyethyleneimine(PEI)arms with candesartan(CD)conjugated via an amide bond was fabricated as a targeted co-delivery nanovector of drug and gene for potential cancer ***,CD was utilized to specifically bind to overexpressed angiotensin II type 1 receptor(AT1R)of tumor cells,strengthen endosomal buffering capacity of CPC and suppress tumor *** self-assembled CPC/p DNA complexes exhibited desirable andhomogenous particle size,moderate positive charges,superior stability,and efficient release of drug and gene in *** cytometry and confocal laser scanning microscopy analyses confirmed that CD-targeted function and CD-enhanced buffering capacity induced high transfection,specific cellular uptake and efficient intracellular delivery of CPC/p DNA complexes in AT1R-overexpressed PANC-1 *** addition,CPC/wt-p53 complexes co-delivering CD and wild type p53(wt-p53)gene achieved synergistic angiogenesis suppression,as compared to mono-delivery and mixed-delivery *** findings suggested that CPC could be an ideal tumor-targeting nanovector for simultaneous transfer of drug and gene.
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