Objective To induce islet allograft long-term survival through cotransplantation of islet cells with sertoli *** Testicular sertoli cells were prepared by digestion with collagenase, trypsin and DNase, and were cultu...
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Objective To induce islet allograft long-term survival through cotransplantation of islet cells with sertoli *** Testicular sertoli cells were prepared by digestion with collagenase, trypsin and DNase, and were cultured for 48 hours. Collagenase digested and Ficoll purified donor (Wistar rat) islets were cotransplanted with allogeneic sertoli cells in the absence of systemic immunosuppression. Terminal leoxynucleotidyl transferase-mediated X-dUTP nick-end labeling (TUNEL) was used to label apoptosis of lymphocytes surrounding the islet *** Cotransplantation of islets and 1 × 107 sertoli cells reversed the diabetic state for more than 60days in 100% (6/6) of the chemically diabetic Sprague Dawley rats. Grafts consisting of islets alone or islets plus 1 × 105 sertoli cells survived only for 5 - 6 days. Apoptosis of lymphocytes surrounding the islets was quite *** Cotransplantation of islets with FasL+ sertoli cells induces local immune privilege and allows long-term graft survival without systemic immunosuppression.
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