Objective： To detect the expression of survivin in subtypes of lymphoma and its value in classifying subtypes of lymphoma. Methods： Paraffin-embedded samples collected from 219 cases of lymphoma and 13 cases of lymp...
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Objective： To detect the expression of survivin in subtypes of lymphoma and its value in classifying subtypes of lymphoma. Methods： Paraffin-embedded samples collected from 219 cases of lymphoma and 13 cases of lymph node reactive proliferation from affiliated hospitals of Sun Yat-sen University during 2001-2003 were examined for the expression of survivin by using immunohistochemical staining. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the mRNA expression of survivin in K562, HL60, Raji, and Jurkat cell lines. Semi-quantitative assay was used to evaluate the quantity of survivin protein and mRNA expression in subtypes of lymphoma. Results： Protein expression of survivin was high and strong in diffuse large B-cell lymphoma （DLBL） （88.6%, 70/79）, Burkitt lymphoma （BL） （100%, 2/2）, and lymphoblastic lymphoma （LBL） （92.3%, 12/13）, while their expression was always lower and weaker in follicular lymphoma （FL） （18.2%）, extranodal marginal zone B-cell lymphoma of mucosaassociated lymphoid tissue （MALT lymphoma） （40.9%） and marginal zone lymphoma （MZL） （33.3%）. There was a significant difference between the higher expression group （DLBL, BL and LBL） and lower one （FL, MZL, and MALT） in the expression of survivin （Chi-square test, X^2=24.77, P〈0.01）. Almost all of Reed-Sternberg cells （R-S cells） in Hodgkin lymphoma （HL） strongly expressed survivin. The protein expression of survivin was positively correlated with mRNA （r=0.6270, P〈0.01）. Conclusion： The expression level of survivin mRNA and protein shows significant difference in subtypes of lymphoma. The expression of survivin mRNA might act as a biomarker to classify the subtypes of lymphoma.
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